Registration Dossier

Administrative data

Endpoint:
immunotoxicity
Remarks:
subchronic
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
other: not rated (non-standard test system)

Data source

Reference
Reference Type:
publication
Title:
Silver-nanoparticle exposure on immune system of mice depending on the route of administration
Author:
Kalmantaeva, O.V. et al.
Year:
2014
Bibliographic source:
Nanotech. Russia 9, 9-10

Materials and methods

Principles of method if other than guideline:
subchronic (intragastral, inhalation, subcutaneous )
dose: 0.1 mg/kg bw silver nanoparticles (AgNPs)
Exposure: 28 days (daily)
AgNPs were applied daily to three groups of female mice (15 animals each) by intragastral, inhalation and subcutaneous (s.c.) administration of a dose of 0.1 mg/kg bw for 28 days. The control group received physiological solution (not further defined). After euthanasia on the 29th day of the experiment, lungs, liver, kidneys, spleen, inguinal lymph nodes, and blood serum were studied using histologic and immunologic methods.
The cytotoxicity of the Argovit preparation was studied in vitro on splenocyte cultures obtained from experimental animals using the MTT method.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: nanomaterial
Details on test material:
Argovit preparation, polyvinylpyrrolidone-coated AgNPs, average particle diameter 43.6±10.7 nm (AFM) Source: Vektor-VIta (Russia)

Test animals

Species:
mouse
Strain:
Balb/c
Sex:
female

Results and discussion

Effect levels

Dose descriptor:
other: General findings
Basis for effect level:
other: see 'Remark'
Remarks on result:
not measured/tested
Remarks:
Effect level not specified (migrated information)

Applicant's summary and conclusion