Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, non guideline study, published in peer reviewed literature, limitations in reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
publication
Title:
Comparison of the Toxicity Thresholds of Water Pollutants to Bacteria, Algae, and Protozoa in Cell Multiplication Inhibition Test
Author:
Bringmann, G. and Kuhn, R.
Year:
1980
Bibliographic source:
Water Research 14(3) 231-241

Materials and methods

Principles of method if other than guideline:
The effect of the test substance on the inhibition of the multiplication of bacterial cells of the genus Pseudomonas was investigated. Cultures of the test organisms were exposed to a series of test concentrations of the test substance for 16 hours and the effects on cell multiplication measured using a turbidimetric technique. Results are reported relative to the blank controls.
GLP compliance:
no
Remarks:
predates GLP

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetic acid
EC Number:
200-580-7
EC Name:
Acetic acid
Cas Number:
64-19-7
Molecular formula:
C2H4O2
IUPAC Name:
acetic acid
Details on test material:
No data reported
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data reported

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
The concentration of the bacterial suspension was measured turbidimetrically at 0 and 16 hours

Test solutions

Vehicle:
no
Details on test solutions:
Test solutions prepared from a stock solution, with each dilution containing 1 part v/v test substance solution in 2^0 to 2^14 v/v double distilled water. Nutrient medium, trace element solution and vitamin solution were added to each test vessel together with 10ml of bacterial suspension.

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Stock cultures of the test strain kept on the nutrient for stock and preliminary cultures in agar slant tubes. New stock cultures prepared at 1 week intervals. The inoculated stock culture incubated at 25°C for 24h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Post exposure observation period:
None

Test conditions

Hardness:
No data reported
Test temperature:
25°C
pH:
authors state that the solution of test substance was neutralised by the addition of a minimal volume of acid or alkaline solution
Dissolved oxygen:
No data reported
Salinity:
Not applicable
Nominal and measured concentrations:
No data reported
Details on test conditions:
Four parallel dilution series in 300ml Erlenmeyer flasks, stoppered with cotton-lined plastic caps. Both inoculated and non-inoculated dilution series left at 25°C for 16h
Reference substance (positive control):
no
Remarks:
No data reported

Results and discussion

Effect concentrations
Duration:
16 h
Dose descriptor:
NOEC
Effect conc.:
1 150 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Details on results:
After 16 hours the extinction of the monochromatic radiation at 436nm in a 10mm layer in the inoculated dilution series was measured. The lowest concentration having a mean extinction value ≥3 below the mean for non-toxic dilutions is reported as the EC3.
Results with reference substance (positive control):
No data reported
Reported statistics and error estimates:
No data reported

Applicant's summary and conclusion

Validity criteria fulfilled:
no
Remarks:
non-standard test
Conclusions:
An 16h EC3 of 850mg/l has been reported for Pseudomonas putida . This can be considered to be the NOEC.
Executive summary:

An 16h EC3 of 850mg/l has been reported for Pseudomonas putida . This can be considered to be the NOEC. Although this study is non-GLP and is non guideline, it is considered suitable for use as a key study. The study investigates the effects of the test substance on a standard test organism. A series of exposure concentrations were used and results reported as the inhibition of culture growth relative to controls.