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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
4-18 April 1995
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: The study report has not been received and therefore cannot be reviewed and evaluated in full.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
Principles of method if other than guideline:
No data reported.
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
TEM 123 (CEL 350 20 H SL)
IUPAC Name:
TEM 123 (CEL 350 20 H SL)
Details on test material:
10% acetic acid. Liquid. Water soluble concentrate (ready for use). Batch number: 0395. Substance was stable under test conditions.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data reported.

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
Assessment of toxicity effects to earthworms were carried out on days 7 and 14.

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
Artificial soil was prepared using: 10% Sphagnum peat, 20% Kaolin clay, 69% industiral sand, 1% calcium, deionised water (to water content of 25%).

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
Species: Eisenia foetida (Savigny) and Eisenia fetida andrei (Bouche) - 1826 (Earthworms). At test initiation: adult worms, 7 months old. Weight at test initiation: 3378-3660 mg/ 10 earthworms. Source: worms were taken from a healthy stock within the test facility, which were kept under laboratory conditions. Acclimitisation: 24 ours in moist artificial soil.

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Post exposure observation period:
No data reported.

Test conditions

Test temperature:
20 +/- 2˚C
pH:
No data reported.
Moisture:
25%
Details on test conditions:
Tests were carried out in 1L glass vessels with lids in a climate controlled chamber. The test chambers were continually exposed to light at 400-800 lux (daylight). Number of worms per vessel: 10. Number of replicates: 4.
Nominal and measured concentrations:
Nominal concentrations: 0, 2.86, 28.6, 71.4, 143, 286 mL/kg dry weight. These nominal concentrations correspond to field doses of: 0, 2000, 20000, 50000, 100000, 200000 L/ha.
Reference substance (positive control):
yes
Remarks:
100% 2-chloracetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
28.6 other: mL/kg d.w.
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 2914 mg as/kg d.w.
Duration:
14 d
Dose descriptor:
LOEC
Effect conc.:
71.4 other: mL/kg d.w.
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 7286 mg as/kg d.w.
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
71.4 - 143 other: mL/kg d.w.
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 7286-14571 mg as/kg d.w.
Details on results:
The test substance was found not to have had a negative effect on the body weight of surviving eatrhworms. There was a statistically significant increase in body weight of the surviving earthworms at the 28.6 mL/ kg d.w. concentration.
Results with reference substance (positive control):
No data reported.
Reported statistics and error estimates:
No data reported.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 14-day LC50 for earthworms of TEM 123is 71.4-143 mL/kg of dry artificial soil based on nominal concentrations. This converts to 7286-14571 mg as/kg of dry artifical soil.
Executive summary:

Lang (1995) has been given a Klimisch score of 4 - not assignable (Klimisch et al, 1996) as the study has not been received and therefore has not been properly evaluated. A summary of the study is included in the EU Draft Assessment Report for Acetic Acid (August 2008).