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Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: The study report has not been received and therefore cannot be reviewed and evaluated in full.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: OECD 208B (Draft 2000): Vegetative vigour
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetic acid
EC Number:
200-580-7
EC Name:
Acetic acid
Cas Number:
64-19-7
Molecular formula:
C2H4O2
IUPAC Name:
acetic acid
Details on test material:
Purity: 100%. Lot/batch#: K26932866
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no further details

Sampling and analysis

Details on sampling:
At the end of the test, final shoot dry weight, plant height and measurements of phytotoxicity were taken.

Test substrate

Details on preparation and application of test substrate:
Agricultural soil from site Cunnersdorf (batch 99) (stored for at least 1 year before used in the test). Sandy Loam. pH-value: 5.7. Corg: 0.99%. Humus content: 1.7%. Water holding capacity (WCmax)(g/100g dry soil): 30. Particl size: 2-0.063mm (sand): 44%; 0.063-0.002mm (silt): 46%; <0.002mm (clay): 10%; <0.02mm (washed out particle): 35%.

Test organisms

open allclose all
Species:
Zea mays
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Batch: 6222969
Species:
Lolium perenne
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Batch: NL 739760-1
Species:
Cucumis sativus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Batch: 600718
Species:
Lycopersicon esculentum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Batch: 627018
Species:
Pisum sativum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Batch: 144022
Species:
Sinapis alba
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Batch: D 350 FS 92

Study design

Test type:
vegetative vigour test
Study type:
laboratory study
Substrate type:
natural soil
Total exposure duration:
21 d

Test conditions

Test temperature:
13-25°C
Details on test conditions:
Relative humidity: 50-95%. Illumination: Light period: 16h (including 8h HQL-light). Light intensity: day light + 8h 11000 1x HQL-light. In life dates: 29 November 2000 - 24 January 2001
Nominal and measured concentrations:
Nominal application rates of 2, 3.6, 6.8, 12, 22 and 40 L acetic acid/ha in 400 L water/ha

Results and discussion

Effect concentrationsopen allclose all
Species:
Zea mays
Duration:
21 d
Dose descriptor:
ER50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Zea mays
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Zea mays
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Zea mays
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
22 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Lolium perenne
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Lolium perenne
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Lolium perenne
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Lolium perenne
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
22 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Cucumis sativus
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Cucumis sativus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Cucumis sativus
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Cucumis sativus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
22 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Sinapis alba
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
37.4 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Sinapis alba
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
< 2 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Sinapis alba
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
32.7 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Sinapis alba
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
6.8 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Lycopersicon esculentum
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
35.4 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Lycopersicon esculentum
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
6.8 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Lycopersicon esculentum
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Lycopersicon esculentum
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
22 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Pisum sativum
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Pisum sativum
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Dry weight
Species:
Pisum sativum
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Species:
Pisum sativum
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
> 40 other: L product/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
Plant height
Details on results:
Recorded effects were used toc alculate EC50 values, LOEC and NOECs. The 21d LC50s for all species were >40 L product /ha.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
No data

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
Sinapsis alba and Lycopersicon esculentum were the two most sensitive species.
Executive summary:

Sinapsis alba and Lycopersicon esculentum were the two most sensitive species. This study followed a standard guideline and was GLP compliant.