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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Abstract, test duration 24 h instead of 48 h.
Principles of method if other than guideline:
Method: DIN 38412 part 11
GLP compliance:
no
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
- Dispersion: saturated solution
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Daphnia magna
- Strain: Daphnia magna (Strauss)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
none
Reference substance (positive control):
not specified
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
>= 27.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 27.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Test parameter: immobilization
- Behavioural abnormalities: no
Validity criteria fulfilled:
not specified
Conclusions:
The EC50 (24h) based on nominal concentration was determined as > 27.6 mg/L indicating that the test substance is not harmful to aquatic invertebrates.
Executive summary:

An acute toxicity study with Daphnia magna was performed according to the DIN 38412 part 11 method, under static conditions (24 h incubation period). An analytical monitoring was included. No toxic reference or control treatment group was included in the test. There was no immobilization, toxic effects or abnormal responses. Thus the EC50 (24 h) was >27.6 mg dodecanedioic acid /L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: No analytic was performed. Nominal test concentrations exceeded the solubility of the test item, test duration 24 h instead of 48 h.
Principles of method if other than guideline:
Static screening method
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Daphnia magna (Strauss)
- Strain: strain identical with the strain of the Bundesgesundheitsamt/Inst. Wasser-Boden-Luft
- Source: own breed
- Feeding during test: no
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
24 h
Post exposure observation period:
none
Hardness:
about 14 ºdH
Test temperature:
Room temperature
Nominal and measured concentrations:
Nominal: 1000 mg/l
Details on test conditions:
- Test vessel: glass beaker, covered with glass plate, 20 ml liquid volume
- Number of daphnids per vessel: about 20
- Photoperiod: about 16 hours illumination per day
- Food type: Chlorella kessleri
- Number of replicates: 1
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Test parameter: immobilization
- Behavioural abnormalities: no
Validity criteria fulfilled:
not specified
Conclusions:
The EC50 (24h) based on nominal concentration was determined as > 1000 mg/L indicating that the test substance is not harmful to aquatic invertebrates.
Executive summary:

An acute toxicity study with Daphnia magna was performed according to the screening method (differing from OECD-Guideline 202, part 1 in: number of daphnids per concentration and fact that the substance concentration was not followed by chemical analysis), under static conditions (24 h incubation period). About 20 daphnids in one replicate were exposed to 1000 mg dodecanedioic acid /L nominal concentration. No toxic reference or control treatment group was included in the test. No analytical monitoring was included. There was no immobilization, toxic effects or abnormal responses. Thus the EC50 (24 h) was >1000 mg dodecanedioic acid /L.

Description of key information

An acute daphnia test (24 h) conducted by Scholz N. (1988) studied the toxicity of dodecanedioic acid on Daphnia magna (determined in a static test according to the DIN 38412 part 11 method). The second acute toxicity study conducted by Wind T. and Mühlberg B. (1982) with Daphnia magna (about 20 daphnids in 1000 mg dodecanedioic acid /L nominal concentration) was performed according to the screening method (differing from OECD-Guideline 202), under static conditions (24 h incubation period) with one replicate per concentration level. 

Key value for chemical safety assessment

Additional information

The results are summarised in the following table:

 Species Endpoint (exposure duration) Exposure concentration  Reference
 Daphnia magna  EC50 (24 h)  > 27.6 mg/L  Scholz N. (1988)
 Daphnia magna  EC50 (24 h)  > 1000 mg/L  Wind T., Mühlberg B. (1982)

The available studies indicate that dodecanedioic acid concentrations up to 1000 mg/L are not toxic to aquatic invertebrates. However, taking into considaration that no analytical monitoring was conducted and that the water solubilty is between 30 mg/L and 40 mg/L the endpoint that should be considered for risk assessment purposes is >27.6 mg/L.