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EC number: 201-291-9 | CAS number: 80-56-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well described study giving reliable information on several terpenes in vivo metabolism in rabbit.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 981
Materials and methods
- Objective of study:
- metabolism
- Principles of method if other than guideline:
- Albino rabbits were orally administered test item and urine was collected for 3 days for identification of urinary metabolites.
- GLP compliance:
- no
Test material
- Reference substance name:
- Pin-2(3)-ene
- EC Number:
- 201-291-9
- EC Name:
- Pin-2(3)-ene
- Cas Number:
- 80-56-8
- Molecular formula:
- C10H16
- IUPAC Name:
- 2,6,6-trimethylbicyclo[3.1.1]hept-2-ene
- Test material form:
- liquid
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rabbit
- Strain:
- other: albino (Japanese White)
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Miyamoto Jikken Dobutsu, Hiroshima, Japan
- Weight at study initiation: 2-3 kg
- Fasting period before study: for 2 days before experiment
- Individual metabolism cages: yes
- Diet (e.g. ad libitum): Oriental RC-4, ad libitum
- Water (e.g. ad libitum): ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 100 mL water containing 0.1 g Tween 80
- Details on exposure:
- Rabbits were administered 20 mL solution through stomach tube followed by 20 mL water, corresponding to 400-700 mg/kg bw.
- Duration and frequency of treatment / exposure:
- Once
Doses / concentrations
- Remarks:
- Doses / Concentrations:
400-700 mg/kg bw
- No. of animals per sex per dose / concentration:
- 6
- Control animals:
- no
- Positive control reference chemical:
- None
- Details on study design:
- None
- Details on dosing and sampling:
- The urine was collected daily for 3 days after drug administration and stored at 0-5°C until time of analysis.
Extraction of urinary metabolites:
The urine was adjusted to pH 4.7 with acetate buffer and incubated with beta-glucuronidase-arylsulfatase (3 mL/1000 mL of the fresh urine) at 37°C for 48 h, followed by continuous ether extraction for 48 h. The ether extracts were washed with 5% NaHCO3 and 5% NaOH to remove the acidic and phenolic fractions, respectively, and dried (magnesium sulfate). Ether was evaporated under reduced pressure to give neutral metabolites. The neutral metabolites were chromatographed on a column containing 100 g of silicic acid (200 mesh). Elution was started with n-hexane, and n-hexane-ethyl acetate mixtures (95:5, 90:10, 85:15, 70:30, and 50:50) were used as subsequent eluents. The acidic metabolites were recovered from the sodium bicarbonate layer by acidification with 5% HCl, followed by ether extraction. The ether extracts were esterified with diazomethane in ether or with dimethyl sulfate in the presence of potassium carbonate in anhydrous acetone. These esters of the acidic metabolites also were chromatographed in the same manner as the neutral metabolites.
Identification of urinary metabolites:
Purification by silicic acid gave pure metabolites. When necessary, metabolites were isolated by preparative TLC or GLC. Structure determination or identification was based on spectral data and chemical transformations. - Statistics:
- None
Results and discussion
- Preliminary studies:
- None
Toxicokinetic / pharmacokinetic studies
- Details on absorption:
- None
- Details on distribution in tissues:
- None
- Details on excretion:
- None
Metabolite characterisation studies
- Metabolites identified:
- yes
- Details on metabolites:
- The main urinary metabolite from (+)-, (-)-, and (+/-)-alpha-pinenes was (-)-trans-verbenol.
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Conclusions:
- The main urinary metabolite from (+)-, (-)-, and (+/-)-alpha-pinenes was (-)-trans-verbenol.
- Executive summary:
The biotransformation of (+)-, (-)-, and (+/-)-alpha-pinene was studied in albino rabbits orally administered 400-700 mg/kg bw of the respective alpha-pinenes in water with 0.1% Tween 80. Urine was collected daily for 3 days and urinary metabolites were identified. In this study, the main urinary metabolite from (+)-, (-)-, and (+/-)-alpha-pinenes was (-)-trans-verbenol.
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