Pin-2(3)-ene

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Remarks:

Ready biodegradability test according to OECD 301 D used to derive PNEC microorganisms.

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

other: OECD 301 D study

Qualifier:

according to guideline

Guideline:

other: OECD 301 D

Deviations:

no

Principles of method if other than guideline:

See cross-referenced study

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on sampling:

Determinations of the concentration of dissolved oxygen using an oxygen electrode in duplicate vessels
were made at day 0, 7, 14, 21, 29, 35, 42, 49 and 56 for the “test suspension” and “inoculum blank with
silica gel” series, at day 0, 7, 14, 21 and 29 for the “procedure control” and “inoculum blank without silica
gel” series, and only at the start of the test and after 7 and 14 days of incubation for the “toxicity control”
series.
Temperature: Measured continuously in the growth chamber

Details on test solutions:

See cross-referenced study

Test organisms (species):

aerobic microorganisms

Details on inoculum:

Origin: River water was sampled from “L’Adour”, situated downstream of a plant treating domestic was
tewater (Aureilhan, France).
Pre-conditioning: The river water was aerated for about 7 days before use as inoculum at the test
temperature to reduce endogenous respiration.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

28 d

Post exposure observation period:

None

Hardness:

No data

Test temperature:

between 20.6 and 21.3°C throughout the test

pH:

no data

Salinity:

Not applicable

Conductivity:

No data

Nominal and measured concentrations:

Nominal concentration: 2 mg/L

Details on test conditions:

See cross-referenced study

Reference substance (positive control):

no

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

ca. 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: readily biodegradable at this nominal test concentration

Details on results:

None

Reported statistics and error estimates:

None

Validity criteria fulfilled:

yes

Conclusions:

The test item is considered as readily biodegradable and the tested concentration is taken as NOECmicrooorganism.

Executive summary:

A ready biodegradability study was performed according to OECD Guideline for Testing of Chemicals No. 301 D, "Ready biodegradability – Closed Bottle Test" (1), referenced as Method C.4-E of Commission Regulation No. 440/2008 amended by Commission Regulation (EU) 2016/266 (2) and with ISO 10634 (7).

Ten BOD bottles were filled with mineral medium inoculated with river water and test item added at a nominal concentration of 2 mg/L with the help of silica gel.

Therefore, the tested concentration of 2 mg/L can be taken as conservative NOECmicrorganism.

Description of key information

The tested concentration in the ready biodegradability study (2 mg/L) is taken as conservative NOECmicrorganism.

Key value for chemical safety assessment

EC50 for microorganisms:

2 mg/L

Additional information

A ready biodegradability study was performed according to OECD Guideline for Testing of Chemicals No. 301 D.

The tested concentration of 2 mg/L is taken as conservative NOECmicrorganism and is valid for key value for chemical safety assessment.