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EC number: 218-760-9 | CAS number: 2226-96-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Original reference not available
Data source
Reference
- Reference Type:
- publication
- Title:
- Antiproliferative effect of piperidine nitroxide TEMPOL on neoplastic and nonneoplastic mammalian cell lines
- Author:
- Gariboldi, M.B., Lucchi, S., Caseri, C., Supino, R., Oliva, C., Monti, E.
- Year:
- 1 998
- Bibliographic source:
- Free Rad. Biol. Med., 24, 913-923
Materials and methods
- Type of study / information:
- antiproliferative activity
Test material
- Reference substance name:
- 4-hydroxy-2,2,6,6-tetramethylpiperidinoxyl
- EC Number:
- 218-760-9
- EC Name:
- 4-hydroxy-2,2,6,6-tetramethylpiperidinoxyl
- Cas Number:
- 2226-96-2
- Molecular formula:
- C9H18NO2•
- IUPAC Name:
- 1-λ1-Oxidanyl-2,2,6,6-tetramethylpiperidin-4-ol
Constituent 1
Results and discussion
Any other information on results incl. tables
4-Hydroxy TEMPO was significantly more toxic to tumour cells than to the corresponding normal cells. No difference in cytotoxicity was observed between cell lines with a multidrug resistant phenotype and the corresponding parental cell lines or between estrogen sensitive and insensitive cell lines. After 2 h exposure no cytotoxic effect was observed, after 24 h irreversible cell damage occurred, that increased with increasing exposure time. The hydroxylamine was significantly less cytotoxic. The cytotoxic action was inhibited by co-exposure to acetylcysteine. Exposure to TEMPOL for 24 h induces a dose dependent accumulation of cells in the G1 phase, upon longer exposure times an increase in G2/M cells is observed. After 24 h exposure to 2.5 mM TEMPOL no detectable DNA degeneration was observed in HBL-100 cells, whereas in MCF-7WT cells internucleosomal DNA fragmentation indicative of apoptosis was observed.
Applicant's summary and conclusion
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