Registration Dossier

Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Original reference not available

Data source

Reference
Reference Type:
publication
Title:
Antiproliferative effect of piperidine nitroxide TEMPOL on neoplastic and nonneoplastic mammalian cell lines
Author:
Gariboldi, M.B., Lucchi, S., Caseri, C., Supino, R., Oliva, C., Monti, E.
Year:
1998
Bibliographic source:
Free Rad. Biol. Med., 24, 913-923

Materials and methods

Type of study / information:
antiproliferative activity

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Any other information on results incl. tables

4-Hydroxy TEMPO was significantly more toxic to tumour cells than to the corresponding normal cells. No difference in cytotoxicity was observed between cell lines with a multidrug resistant phenotype and the corresponding parental cell lines or between estrogen sensitive and insensitive cell lines. After 2 h exposure no cytotoxic effect was observed, after 24 h irreversible cell damage occurred, that increased with increasing exposure time. The hydroxylamine was significantly less cytotoxic. The cytotoxic action was inhibited by co-exposure to acetylcysteine. Exposure to TEMPOL for 24 h induces a dose dependent accumulation of cells in the G1 phase, upon longer exposure times an increase in G2/M cells is observed. After 24 h exposure to 2.5 mM TEMPOL no detectable DNA degeneration was observed in HBL-100 cells, whereas in MCF-7WT cells internucleosomal DNA fragmentation indicative of apoptosis was observed.

Applicant's summary and conclusion