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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

additional toxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Original reference not available

Data source

Reference Type:
Antiproliferative effect of piperidine nitroxide TEMPOL on neoplastic and nonneoplastic mammalian cell lines
Gariboldi, M.B., Lucchi, S., Caseri, C., Supino, R., Oliva, C., Monti, E.
Bibliographic source:
Free Rad. Biol. Med., 24, 913-923

Materials and methods

Type of study / information:
antiproliferative activity

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:

Results and discussion

Any other information on results incl. tables

4-Hydroxy TEMPO was significantly more toxic to tumour cells than to the corresponding normal cells. No difference in cytotoxicity was observed between cell lines with a multidrug resistant phenotype and the corresponding parental cell lines or between estrogen sensitive and insensitive cell lines. After 2 h exposure no cytotoxic effect was observed, after 24 h irreversible cell damage occurred, that increased with increasing exposure time. The hydroxylamine was significantly less cytotoxic. The cytotoxic action was inhibited by co-exposure to acetylcysteine. Exposure to TEMPOL for 24 h induces a dose dependent accumulation of cells in the G1 phase, upon longer exposure times an increase in G2/M cells is observed. After 24 h exposure to 2.5 mM TEMPOL no detectable DNA degeneration was observed in HBL-100 cells, whereas in MCF-7WT cells internucleosomal DNA fragmentation indicative of apoptosis was observed.

Applicant's summary and conclusion