2-(octylthio)ethanol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

01-1957 to 03-1957

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Data included are from range-finding tests. Limited information on the test substance, animals tested/environmental conditions and details on examinations performed were provided. Animals were not fasted prior to testing. Complete necropsies were not performed for all animals. Not GLP.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1957

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: recommendations outlined by the National Safety Council

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

2-(octylthio)ethanol

EC Number:

222-598-4

EC Name:

2-(octylthio)ethanol

Cas Number:

3547-33-9

Molecular formula:

C10H22OS

IUPAC Name:

2-(octylsulfanyl)ethan-1-ol

Constituent 2

Reference substance name:

Ethanol, 2-(octylthio)-

IUPAC Name:

Ethanol, 2-(octylthio)-

Constituent 3

Reference substance name:

2-hydroxyethyl-n-octyl sulfide

IUPAC Name:

2-hydroxyethyl-n-octyl sulfide

Details on test material:

- Name of test material (as cited in study report): 2-Hydroxyethyl-n-octyl sulfide
- Substance type: active
- Physical state: colorless liquid

Test animals

Species:

rat

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 108-127
- Fasting period before study: No

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other:

Details on oral exposure:

VEHICLE
- Amount of vehicle (if gavage): The test substance was diluted with Mazola and the concentrations were adjusted for reasonable accuracy in volume measurement so that at least 1 mL was administered at the lowest dosage. The low order of toxicity made it necessary to administer the undiluted test substance at the highest dosage.
- Justification for choice of vehicle: Preliminary tests indicated that the test substance was readily miscible with Mazola.


MAXIMUM DOSE VOLUME APPLIED: At least 1 mL was administered at the lowest dosage level.

Doses:

3.98, 7.95, 15.80, 31.60 g/kg

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weights on the animals were reported at the beginning of the study and after 14 days.
- Necropsy of survivors performed: yes; All fatalities were autopsied to exclude extraneous causes of death. Some survivors were autopsied for gross lesions.
- Other examinations performed: clinical signs, body weight and autopsies of gross lesions

Statistics:

LD50 was estimated by Thompson's method of moving averages using the tables of Weil.

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

8 530 mg/kg bw

95% CL:

>= 6 040 - <= 12 050

Mortality:

All of the five rats dosed with the undiluted test substance at 31.60 g/kg died overnight. Three of the five rats on the 15.80 g/kg level died overnight; the two remaining rats died on the second day. Two rats on the 7.95 g/kg level died on the second day.

Clinical signs:

other: Immediately following the oral administration of the test substance, the rats on the three highest dosage levels were sick and ataxic. The surviving rats on the 7.95 g/kg and 3.98 g/kg levels were sluggish the first day, but recovered fully thereafter.

Gross pathology:

Autopsies of the surviviors revealed no gross lesions.

Applicant's summary and conclusion

Conclusions:

Based on these test conditions it has been determined that the LD50 is 8.53 g/kg for rats when exposed via oral gavage.

Executive summary:

The LD50 of ethanol, 2-(octylthio) was determined by following the recommendations outlined by the National Safety Council. Five male rats were dosed by oral gavage at 3980, 7950, 15800 and 31600 mg/kg bw. Immediately following dosing, rats in the 31600 mg/kg bw group were sick and ataxic; all 5 rats died overnight. Of the 5 rats administered 15800 mg/kg bw, 3 died overnight; the 2 remaining rats died on the second day. Two rats in the 7950 mg/kg dose group died on the second day. The surviving rats in the 7950 and 3980 mg/kg groups were sluggish the first day, but recovered fully thereafter and showed good weight gains during the 14-day observation period. Autopsies of survivors revealed no gross lesions. The LD50 was 8530 mg/kg bw.