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EC number: 247-975-0 | CAS number: 26760-64-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, no restrictions, fully adequate for assessment
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
- Reference Type:
- publication
- Title:
- Genetic toxicology testing of 41 industrial chemicals
- Author:
- Dean BJ, Brooks TM, Hodson-Walker G and Hutson DH
- Year:
- 1 985
- Bibliographic source:
- Mutation Research 153:57- 77
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-methyl-2-butene
- IUPAC Name:
- 2-methyl-2-butene
- Reference substance name:
- 2-methylbut-2-ene
- EC Number:
- 208-156-3
- EC Name:
- 2-methylbut-2-ene
- Cas Number:
- 513-35-9
- IUPAC Name:
- 2-methylbut-2-ene
- Details on test material:
- - Name of test material (as cited in study report): 2-methyl-2-butene
- Supplier: Shell Chemie, Rotterdam, The Netherlands
- Batch number: Indent 9200/9579
- Purity: 84.9%
- Composition of test material, percentage of components: 2-methylbut-2-ene 84.9%, 3-methylbut-1-ene 7.3%, isopentane 3.6%, n-pentane 1.0%, trans pent-2-ene 1.2%, cis pent-1-ene 0.3%, pent-1-ene 0.2%, 3-methylpentane + trans 1,3-pentadiene 0.3%, tert. amyl alcohol 0.4%, total C4 compounds 0.2%, polymers 0.1%
- Expiration date of the lot/batch: 1 January 1981
-Storage conditions: in the dark at 0-5°C
Constituent 1
Constituent 2
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium, other: TA1538
- Species / strain / cell type:
- E. coli WP2
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S-9
- Test concentrations with justification for top dose:
- 31.25, 62.5, 125, 250, 500, 1000, 2000 and 4000 µg/plate
- Vehicle / solvent:
- - ethanol
Stability of dosing solutions: solutions of 2-methyl-2-butene in absolute ethanol were considered to be stable for at least 1 day.
Controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol
- Positive controls:
- yes
- Positive control substance:
- other: benzo(a)pyrene, 4-nitroquinoline-N-oxide, sodium azide, neutral red
- Remarks:
- benzo(a)pyrene (TA1538,TA98, TA100); neutral red (TA1537), 4-nitroquinoline-N-oxide (E.coli strains) and sodium azide (TA1535)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) with pre-incubation
DURATION
- Preincubation period: 30 minutes
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS:
- Three plates per dose level using sealed containers
-Two replicate assays were performed on different days
DETERMINATION OF CYTOTOXICITY
- Relative total growth - Evaluation criteria:
- Data are interpreted on the basis of a consistent doubling of the spontaneous reversion frequency confirmed by a dose-response relationship. Where the number of induced revertants is less than twice the spontaneous rate, but a reproducible dose-related increase in revertants is detected, this is interpreted as a positive response.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium, other: TA538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
2-methyl-2-butene was not mutagenic in any of the five strains of Salmonella or in the two strains of E. coli tested in the presence or absence of metabolic activation. - Executive summary:
2 -methyl-2 -butene was tested in an Ames assay in 5 strains of Salmonella typhimurium (TA1535, TA 1537, TA1538, TA 100, and TA98) and in 2 strains of Escherichia coli (WP2 and WP2uvrA) in the presence and absence of rat liver S-9. Five dose levels were tested, with three plates per dose level using sealed containers. Concurrent positive and solvent controls were also tested with and without metabolic activation (rat liver S9). Two replicate assays were performed on different days to confirm the reproducibility of the results.
2 -methyl-2 -butene was not mutagenic in any of the five strains of Salmonella or in the two strains of E. coli tested in the presence or absence of metabolic activation.
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