1,3-dihydroxyacetone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-04-06 to 1999-04-09

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

adopted 4 April 1984

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Official Journal No. L 383 A, 29 December 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dihydroxyacetone

EC Number:

202-494-5

EC Name:

1,3-dihydroxyacetone

Cas Number:

96-26-4

Molecular formula:

C3H6O3

IUPAC Name:

1,3-dihydroxyacetone

Details on test material:

Name: Dihydroxyacetone

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test item (reconstituted water and test item) was freshly prepared. The test item was weighed into a calibrated flask and filled with reconstituted water. After stirring of 10 minutes, the test item was completely dissolved and the flask filled to the mark with reconstituted water.
- Controls: reconstituted water for Daphnia (ELENDT M4)
- Concentration of the test item in the test medium: 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Daphnia magna (Straus)
- Age at study initiation: not older than 24 hours
- Method of breeding: The parent strain (stock) was bred and maintained in vessels containing many daphnids in different ages.
For a new study, young daphnids were separated in 100 mL of reconstituted water. Once a week, the medium was renewed and the daphnids were fed with fresch water algae. Newborn animals were separated and used for the acute immobilization study.
- Source: Test Facility
- Feeding during test : No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/L expressed as CaCO3

Test temperature:

Start of test: 22 °C
End of test: 21 °C

pH:

Start of test: pH 8.0
End of test: pH 7.9 - 8.0

Dissolved oxygen:

Start of test: 96.9 - 99.6 %
End of test: 94.4 - 96.2 %

Nominal and measured concentrations:

Nominal concentration: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 25 mL glass vessel
- Type (delete if not applicable): open
- Volume of solution: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light - 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The mobility of the daphnids was determined after 24 and 48 hours.

RANGE-FINDING STUDY
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilization observed in the pretest with 100 mg/L.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: No
- Immobilisation of control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No

Any other information on results incl. tables

Clinical findings

The nominal concentration and immobilization data of the study are shown in thefollowing table:

nominal concentration g/L	dead or immobilized/ exposed Daphnia magna
	24 hours                            48 hours
Control	0 / 20	0 / 20
0.1	0/20	0/20

Daphnia exposed to the nominal concentration of 0.1 g/L (limit test) were not immobilized.

No remarkable observations were made concerning the appearance of the test material. All test media were clear preparations and stayed unchanged throughout the study.

EC₅₀-value

Under the given experimental conditions, the test material showed a 48 h EC₅₀ value of > 0,1 g/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

OECD 202 validity criteria fulfilled

Conclusions:

Under the given experimental conditions, the 48 h EC50 value of the test item exceeded the limit dose of 100 mg/L

Executive summary:

The objective of this study was to determine the acute toxicity of the test material to Daphnia magna. For this purpose, young Daphnia magna were exposed to an aqueous test material concentration under defined conditions. The study comprised of four vessels per concentration containing five Daphnia magna , i.e. 20 Daphnia per concentration (test medium group). The Daphnia were observed for immobilization up to 48 hours. The study was started with the exposure of Daphnia to the test material concentration of nominal 0.1 g/L in an open system. An analytical determination of the concentration was not performed.

Daphnia exposed to the test item at a nominal concentration of 100 mg/L were not affected.

According to the tested nominal concentrations the following values were obtained

	24h EC50	> 100 mg/L
	48h EC50	> 100 mg/L

Under the given experimental conditions, the test material showed a 48 h EC50 value exceeding the limit dose of 100 mg/L.