1,3-dihydroxyacetone

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999/05/11 - 1999/07/21

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Version / remarks:

29 December 1992

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

adopted 17 July 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,3-dihydroxyacetone

EC Number:

202-494-5

EC Name:

1,3-dihydroxyacetone

Cas Number:

96-26-4

Molecular formula:

C3H6O3

IUPAC Name:

1,3-dihydroxyacetone

Details on test material:

Purity: 99.5 %

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Effluent of an activated sludge plant which is operated with a mixed waste water (industrial and municipal) of Merck KGaA, Darmstadt, Germany
- Method of cultivation: Aeration over 5 days
- Storage length: 5 days
- Preparation of inoculum for exposure: Filtration (coarse filter)
- Concentration of sludge: 5 mL/L
- Initial cell/biomass concentration:
- Water filtered: yes
- Type and size of filter used, if any: coarse folded paper filter

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

4 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Accordung to guideline

Reference substance

Reference substance:

aniline

Results and discussion

Test performance:

According to guideline

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

67

Sampling time:

7 d

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

74

Sampling time:

14 d

% Degradation 3

Key result

Parameter:

% degradation (O2 consumption)

Value:

77

Sampling time:

28 d

Remarks on result:

other: 10-day window passed

Details on results:

Duration of the experiment: 28d
Temperature: 20 +/- 1 DEG

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test material was readily biodegradable under the described conditions of this study.

Executive summary:

In this study, the test item was assessed for its possible biodegradation. For this purpose a screening for ready biodegradability in an aerobic aqueous medium was performed at a concentration of 4 mg/L. The study design follows the conditions as described in the OECD Guideline for testing of chemicals 301D (Closed bottle method).

After 28d, the reference substance (Aniline) shows full degradation suggesting the validity of the experimental setup. For the test item, a degradation of 67% and 77% was obtained after 7 and 28 days, respectively. These experimental values indicate that the test substance is ready biodegradable under the conditions of this experiment.