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Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1971
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Not assignable; Insufficient detail in the IUCLID entry to determine reliability.
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Enzymatic Dechlorination: Dechlorination of Chloroethanes and Propanes in vitro
Author:
van Dyke, R.A., Wineman, C.G.
Year:
1971
Bibliographic source:
Biochem. Pharmacol. 20, 463 - 470

Materials and methods

Type of study / information:
Type: other: Biotransformation
Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
not applicable
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,2-Dichloropropane

Results and discussion

Any other information on results incl. tables

Analysis with rat liver microsomes showed that the enzyme responsible for the dechlorination of 1,2-dichloropropane needs O2 and  NADPH.  Pheno-

barbital and benzpyrene can inducethis enzyme but not methylcholanthrene

Applicant's summary and conclusion

Conclusions:
Analysis with rat liver microsomes showed that the enzyme responsible for the dechlorination of 1,2-dichloropropane needs O2 and NADPH. Phenobarbital and benzpyrene can induce this enzyme but not methylcholanthrene.
Executive summary:

The enzymatic dechlorination of a series of chloroethanes and chloropropanes was investigated. It was found that these materials were dechlorinated enzymatically by an enzyme system located in hepatic microsomes. This system requires NADPH and oxy­gen, and is inducible by phenobarbital and benzpyrene, but not by methylcholanthrene. The pH optimum of this system was found to be 8.2. Evidence is presented that a factor is present in the 105,000 g supernatant which is necessary for optimum activity. This supernatant factor is not inducible. The dechlorination varied depending on the extent of chlorination of the ethane or propane.

Analysis with rat liver microsomes showed that the enzyme responsible for the dechlorination of 1,2-dichloropropane needs O2 and NADPH. Phenobarbital and benzpyrene can induce this enzyme but not methylcholanthrene.