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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report Date:
1985

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Version / remarks:
1981
GLP compliance:
no
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Savo, Ivanovas Kisslegg
- Fasting period before study: on test day
- Housing: individually
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: > 5 days


ENVIRONMENTAL CONDITIONS
according to guideline


Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
according to guideline

REMOVAL OF TEST SUBSTANCE
- Washing: yes
- Time after start of exposure: 24 h


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.00681-0.0681 mL/kg (males), 0.0147-0.0681 mL/kg (females)
- Constant concentration used: yes



Duration of exposure:
24h
Doses:
6.95-69.5 mg/kg bw (males)
15.0-69.5 mg/kg bw (females)
No. of animals per sex per dose:
2-3
Control animals:
not required
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs
Statistics:
Probit Analysis

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
> 6.95 - < 15 mg/kg bw
Sex:
female
Dose descriptor:
LD50
Effect level:
32.2 mg/kg bw
Mortality:
Deaths occurred between 30 min and 2 h after application.
Clinical signs:
decrease of muscle tone, general loss of reflexes, streneous respiration, cyanosis, mydriasis.
First symptoms 3-45 min post appl. persistant 2 to 3 h.
Body weight:
not determined
Gross pathology:
no substance related changes
Other findings:
formation of eschar at the exposed skin site

Any other information on results incl. tables

The test substance was applied undiluted and caused severe local reactions in addition to the systemic toxicity.

Applicant's summary and conclusion

Interpretation of results:
Category 1 based on GHS criteria
Remarks:
Migrated information