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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
2006
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
Concentration of the test substance was determined in all concentration levels at test start and after 72 h.
Vehicle:
no
Details on test solutions:
100 mg/L of the test substance were weighed and mixed with dilution water by agitation.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Test temperature:
22-23°C
pH:
test start: 8.09-8.18
test end: 8.13-9.53
Nominal and measured concentrations:
nominal: 10, 32, 100, 320 and 1000 µg/l
geometric mean of measured concentrations: 5.28, 9.28, 16.3, 30.1 and 395 µg/l
Details on test conditions:
With regard to the volatility of the test substance glass flasks without headspace were used to reduce losses of test item.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
17.3 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 14.9 - 19.9 µg/l
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
34.2 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL 32.0 - 37.6
Results with reference substance (positive control):
EC50 (growth rateh, 72h): 0.69 mg/l (95% CL 0.64-0.74 mg/L)
Reported statistics and error estimates:
EC10- and EC50-values of the growth rate and yield inhibition after 72 h were calculated by sigmoidal dose-response regression. Calculation of the confidence intervals EC10- and EC50-values were carried out using standard procedures according to CLOPPER and PEARSON (1934).
Validity criteria fulfilled:
yes
Conclusions:
In guideline study (GLP study) conducted according to OECD TG 201 with the freshwater alga Desmodesmus subspicatus toxicity of  Acrolein cyanhydrin acetate (ACA) was determined.
The 72h ErC50 based on geometric mean measured concentrations was calculated to be 34.2 µg/l (32.0 - 37.6 µg/l).
The 72h ErC10 based on geometric mean measured concentrations was calculated to be 17.3 µg/l (14.9 - 19.9 µg/l).

Description of key information

In guideline study (GLP study) conducted according to OECD TG 201 with the freshwater alga Desmodesmus subspicatus toxicity of  Acrolein cyanhydrin acetate (ACA) was determined.

The 72h ErC50 based on geometric mean measured concentrations was calculated to be 34.2 µg/l (32.0 - 37.6 µg/l).

The 72h ErC10 based on geometric mean measured concentrations was calculated to be 17.3 µg/l (14.9 - 19.9 µg/l).

Key value for chemical safety assessment

EC50 for freshwater algae:
34.2 µg/L
EC10 or NOEC for freshwater algae:
17.3 µg/L

Additional information