Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1984
Report Date:
1984

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412
Principles of method if other than guideline:
Method: other: DIN 38412
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
560 mg of Acroleincyanhydrin-o-acetat was weighed and dissolved in 10 ml dimethylsulphoxide (DMSO), this solution was further diluted with DMSO. 1 ml of the concentrated solutions were added to 10 l of dilution water to prepare the test solution.

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
Fishes were obtained from a commercial hatchery. Average length and weight were 6.1 +/-0.3 cm and 1.9 +/- 0.3 g.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20 +/- 1°C
pH:
7.2 - 8.0
Dissolved oxygen:
> 6.1 mg/l
Nominal and measured concentrations:
nominal: 0.32, 0.56, 1, 1.8, 3.2, 4.2 and 5.6 mg/l
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
0.56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.75 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (0.56-1.0)
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Reported statistics and error estimates:
LC50 values and their confidence intervals were calculated by means of a parametric model developed by Kooijman (1981).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the results of this study the LC50 is 0.75 mg/L.