1-cyanoallyl acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

All concentration levels and the control were analysed via GC after 0h and 24 h (new media) and after 24h and 48h (old media). Recovery rates in all concentration levels were in the range of 86-100% in the new media and in the range of 55-75% in the old media.

Vehicle:

no

Details on test solutions:

A stock solution (10 mg/l) was prepared by weighing the tests substance and dilution with dilution water. The solution was agitated.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organisms were bred at the testing facility in M4 medium at 20°C with 16h illumination. Test organims were 2-24h old and were not fed during the study.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

19-20°C

pH:

7.6-7.8 after 48h

Dissolved oxygen:

7.7-7.8 after 48h

Nominal and measured concentrations:

nominal: 0.125, 0.250, 0.500, 1.00 and 2.00 mg/l
geometric mean measured concentration: 0.092, 0.193, 0.394, 0.807 and 1.74 mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

0.397 mg/L

Nominal / measured:

meas. (geom. mean)

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

0.312 mg/L

Nominal / measured:

meas. (geom. mean)

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.661 mg/L

Nominal / measured:

meas. (geom. mean)

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.431 mg/L

Nominal / measured:

meas. (geom. mean)

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

1.74 mg/L

Nominal / measured:

meas. (geom. mean)

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

0.807 mg/L

Nominal / measured:

meas. (geom. mean)

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.193 mg/L

Nominal / measured:

meas. (geom. mean)

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

0.394 mg/L

Nominal / measured:

meas. (geom. mean)

Results with reference substance (positive control):

EC 50 (24h): 1.64 mg/l

Reported statistics and error estimates:

EC values were calculated by sigmoidal dose-response regression.

Validity criteria fulfilled:

yes

Conclusions:

In an acute immobilization study according to OECD202 (GLP study) with Daphnia magna conducted under semi-static conditions the toxicity of was determined.
The 48h EC50 based on geometric mean measured concentrations was calculated to be 0.431 mg/l (0.396 -0.470 mg/l).

Description of key information

In an acute immobilization study according to OECD202 (GLP study) with Daphnia magna conducted under semi-static conditions the toxicity of  Acrolein cyanhydrin acetate (ACA) was determined.

The 48h EC50 based on geometric mean measured concentrations was calculated to be 0.431 mg/l  (0.396 -0.470 mg/l).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.431 mg/L