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EC number: 225-642-0
CAS number: 4985-85-7
The method meets the requirements of the OECD Guidelines for Testing of
Chemicals No. 471 "Bacterial Reverse Mutation Test", and No. 472
(Genetic Toxicology: Escherichia coli, Reverse Mutation Assay).
Salmonella typhimurium strains TA1535, TA1537, TA98, TA100 and
Escherichia coli strain WP2 uvrA were treated with N-(3-Aminopropyl)
Diethanolamine using the Ames plate incorporation method at five dose
levels, in triplicate, both with and without the addition of a rat liver
homogenate metabolising system (1 0% liver S9 in standard co-factors).
The dose range was determined in a preliminary toxicity assay and was 50
to 5000 µg/plate in the first experiment. The experiment was repeated on
a separate day using the same dose range as Experiment 1, fresh cultures
of the bacterial strains and fresh test material formulations.
The vehicle (sterile distilled water) control plates gave counts of
revertant colonies within the normal range. All of the positive control
chemicals used in the test induced marked increases in the frequency of
revertant colonies, both with or without metabolic activation. Thus, the
sensitivity of the assay and the efficacy of the S9-mix were validated.
N-(3-Aminopropyl) Diethanolamine caused no visible reduction in the
growth of the bacterial background lawn at any dose level.
N-(3-Aminopropyl) Diethanolamine was, therefore, tested up to the
maximum recommended dose level of 5000 µg/plate. No test material
precipitate was observed on the plates at any of the doses tested in
either the presence or absence of S9-mix.
No significant increases in the frequency of revertant colonies were
recorded for any of the bacterial strains, with any dose of the test
material, either with or without metabolic activation.
N-(3-Aminopropyl) Diethanolamine was considered to be non-mutagenic the
conditions of this test.
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