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EC number: 252-552-9
CAS number: 35415-27-1
Reverse mutation testing of the test material, trinonyl benzene-1,2,4
-tricarboxylate, was conducted according to OECD testing guideline 471
and GLP using 5 strains of bacteria: Salmonella typhimurium TA97a, TA98,
TA100, TA102, and TA1535. The test material (dissolved in DMSO) was
tested up to concentrations of 5000 μg/plate in the absence and presence
of S9-mix (0.74 % final concentration in the treatment) in two
experiments (experiment 1: plate incorporation method; experiment 2:
In all experiments, no precipitation of the test item was observed at
any of the tested concentrations up to 5000 μg/plate. In all three
experiments, the test item showed no cytotoxicity towards all bacteria
strains (no reduction of background lawn and number of revertants). The
confirmation tests of the genotype did not show any irregularities. The
control of the titre was above the demanded value of 10EXP9 bacteria/mL.
All of the means of all replicates of the spontaneous revertants (in
negative and solvent controls) were within the range of the historical
data of the test facility. Nearly all numbers of revertant colonies of
the positive controls were within the range of the historical data of
the laboratory, but all were increased in comparison with the negative
controls, which demonstrated the mutagenic potential of the diagnostic
mutagens. Thus indicating that the test conditions were adequate and
that the metabolic activation system functioned properly. Since all
criteria for acceptability have been met, the study is considered valid.
The test item showed no increase in the number of revertants in all
bacteria strains in both experiments. Based on the results of this study
it is concluded that the test item is not mutagenic in the Salmonella
typhimurium test strains TA97a, TA98, TA100, TA102, and TA1535 in the
absence and presence of metabolic activation under the experimental
conditions in the present study.
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