Trinonyl benzene-1,2,4-tricarboxylate

Endpoint summary

Description of key information

Key values for acute aquatic toxicity

Aquatic plants: 72h-EC50 (green algae, growth rate) > 54 mg/L

Invertebrates: 48h-EC50 (Daphnia magna, immobility) > 108 mg/L

Fish: 96h-LC50 (Oryzias latipes, mortality) > 108 mg/L

In conclusion, no acute aquatic toxicity is expected for the submission substance below its water solubility limit.

Key values for chronic aquatic toxicity

Aquatic plants: 72h-NOECr (green algae, growth rate) = 27 mg/L

Invertebrates: 21d-NOEC (Daphnia magna, reproduction) = 4.4 mg/L

In conclusion, no long-term aquatic toxicity is expected below the water solubility of the submission substance.

Key values for toxicity to microorganisms

Microorganisms: 4.75h-EC10 (Pseudomonas putida, respiration) > 915 - 1060 mg a.i./L

In conclusion, no toxicity to microorganisms is expected below the water solubility of the submission substance.

Additional information

No aquatic toxicity data for the submission substance is available. However adequate and reliable data for structural anologues (i.e. C8TM and C8C10TM, for read-across justification please see read-across report in section 13) are reported below.

Acute aquatic toxicity

Reliable key acute toxicity studies for aquatic plants, invertebrates and fish are available (OECD guideline sudies: MoE Japan, 2001, RL1, analogue substance: trioctyl benzene-1,2,4-tricarboxylate, trioctyl ester, C8TM, CAS no. 89-04-3). In addition, two supporting studies are available for daphnia (Huels AG, 1994, RL2 and ICI GEL, 1990, RL4; analogue substance: 1,2,4-benzenetricarboxylic acid, mixed decyl and octyl triesters, C8C10TM, CAS no. 90218-76-1), and one reliable supporting study is available for aquatic plants and fish (Huels AG, 1994, RL2). All key studies and most of the supporting studies were performed in compliance with GLP. Solubilizers were used to increase the low water solubility of the test substances. Analytically verification of the test concentrations was available. No acute toxicity was observed in algae, daphnia or fish.

Chronic aquatic toxicity

With regard to the toxicity studies on aquatic plants, long-term effects on growth rate were observed after 72 hours of exposure, but not on yield of biomass. The tested concentrations lay above the water solubility of the test substance, except for the lowest of measured concentrations, 0.79 mg/L. No significant adverse effects were observed in concentrations up to 25 mg/L (corresponding to 27 mg/L of the target substance). Test concentrations of the supporting study lay below the water solubility (lowest at nominal 0.24 mg/L), and no effects were observed up to the highest test concentration of 3.5 mg/L (corresponding to 3.3 -3.8 mg/L of the target substance). Therefore, no toxicity to green algae is expected below the water solubility of the submission substances.

Reliable chronic toxicity studies for aquatic invertebrates are available (one key (MoE Japan, 2001, RL1, analogue substance: C8TM) and one supporting study (Huels AG, 1997, RL2, analogue substance: C8C10TM), according to OECD 211). The studies were performed in compliance with GLP. Solubilizers were used to increase the low water solubility of the test substances. Analytically verification of the test concentrations is available. Significant effects on lethality of parent daphnids and reproduction were observed during 21 days of exposure; however, all tested concentrations lay above the water solubility of the test substance. No significant adverse effects were observed in concentrations up to 4.04 mg/L (corresponding to 4.4 of the target substance), and in the limit test of the supporting study at 4.5 mg/L (corresponding to 4.2 -4.9 mg/L of the target substance). Therefore, no long-term toxicity to daphnia is expected below the water solubility of the submission substance.

No long-term toxicity study on fish is available; however, no long-term toxicity to fish is expected because neither short-term toxicity to fish, aquatic invertebrates and plants nor long-term toxicity to aquatic invertebrates was observed below the water solubility of the test substances.

Toxicity to microorganisms

Microorganism toxicity was assessed in a reliable activated sludge respiration inhibition study (Huels Method, Huels AG, 1994, RL2, analogue substance: C8C10TM) and in compliance with GLP. No effect on respiration (biochemical oxygen demand) was observed during 4.75 hours of exposure to three concentrations up to 980 mg a.i./L test substance (nominal 1000 mg/L, use of solubilizer; corresponding to 915 -1060 and 933 -1083 mg/L of the target substance). No analytical verification of the test concentrations was performed.