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EC number: 407-420-7 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
OECD guideline ready biodegradation and inherent biodegradation study (GLP compliant) have shown test material to be not readily and inherently biodegradable respectively.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
A study was performed to assess the ready biodegradability of test substance according to OECD Guideline 301 C.
Activated sludge obtained from a local sewage treatment works was used as the inoculum. The biodegradation was measured over a 28-d period at a nominal rest concentration of 100 mg/L in a manometric respirometer and at the end of the test period the concentration of Dissolved Organic Carbon (DOC) and test substance remaining were determined.
The Biochemical Oxygen Demand (BOD) of test was measured in a respirometric test over 28 d. Only a negligible BOD was found during the 28 d of the test and the BOD5 and BOD28 of the test substance are essentially zero.
The Dissolved Organic Carbon (DOC) level as determined at the end of the rest corresponded to zero biodegradation as did specific analysis. The Chemical Oxygen Demand (COD) was measured as 1.09 g 02/g test substance. The reference substance showed 100 % degradation within 14 d.
The test substance is classified as not readily biodegradable (Brown, D, 1991).
A study was performed to assess the inherent biodegradability of test substance in a Zahn-Wellens test according to Official Journal of the European Communities, L 133, Volume 31 Part C: Methods for the determination of ecotoxicity. Biodegradation: Zahn-Wellens test and similar or equivalent to OECD Guideline 302 B.
Activated sludge obtained from a local sewage treatment works was used as the inoculum. The concentration of dissolved non-purgeable organic carbon (DNPOC) was used to assess the ultimate biodegradation over the 28 d test period and the concentration of test substance was determined to assess any primary biodegradation.
The results for test substance exhibited some variability and ranged from <1 to 8 % biodegradation. As these values appeared to vary in a random fashion (i.e.it was not a gradual rise or an adaptation phase followed by arises) this pattern is considered to be due to the variability of the blank which is regarded as the main contributor to the sensitivity of this method. The reference substance showed 100 % degradation within 14 d.
The test substance is classified as not inherently biodegradable (Comber MHI and Stanley RD, 1995).
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