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EC number: 700-736-9
CAS number: 2149571-40-2
EC50, 48 hours, Daphnia Magna = 0.525 mg/L
NOEC, 48 hours, Daphnia Magna = 0.413 mg/L
An acute toxicity study (Huntingdon Life Sciences, 2015) was conducted
in Daphnia Magna to determine the effects of the test substance MTF in
aquatic invertebrates. The study was conducted according to OECD test
guideline 202 and EC guideline C2, and in compliance with GLP.
Groups of twenty Daphnia, less than 24 hours old, were exposed for 48
hours to MTF, at nominal concentrations of 0.209, 0.460, 1.01, 2.23 and
4.90 mg/L; highest test level approximated the limit of aqueous
solubility of the test substance. Tetrahydrofuran was used as a vehicle
to aid the dissolution of the test substance. The concentrations of THF
in test media were determined by Gas Chromatography-Mass Spectrometry.
Observations of the Daphnia in each control, solvent control and test
vessels were made after approximately 24 and 48 hours. After 48 hours,
the lowest measured concentration resulting in 100% immobility was 0.667
mg/L (mean measured) and the highest concentration at which no
immobilisation occurred was 0.413 mg/L (mean measured). The 48-hour EC50
was calculated to be 0.525 mg/L (95% confidence limits 0.413 - 0.667
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