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EC number: 700-736-9
CAS number: 2149571-40-2
3-Hour EC50 = 928 mg/L based on calculation. EC50 > 1000 mg/L based on
actual percentage inhibition (41% at 1000 mg/L).
3-Hour NOEC = 30 mg/L.
An Activated Sludge Respiration Inhibition Test (ASRIT) was performed
(Huntingdon Life Science, 2013) to assess the effect of MTF on sewage
microorganisms. The study was conducted according to OECD test guideline
209 and in compliance with GLP.
Samples of activated sluge taken from a supply which treats
predominantly domestic waste were exposed to nominal MTF concentrations
10, 30, 100, 300, and 1000 mg/L. The rate of oxygen consumption of these
solutions over three hours were compared to that of a concurrent control
solution; four replicate samples were used for each concentration
including the control.
The mean respiration rates of the sludge exposed to MTF was
significantly decreased by 21% at 100 mg/L, 37% at 300 mg/L and 41% at
1000 mg/L. Based on these data, an EC20 value of 105 mg/L (95%
confidence limits of 28.5 and 388 mg/L) was calculated and the EC50
value was estimated to be 928 mg/L (95% confidence limits of 453 and
1904 mg/L) although based on percentage inhibition, the EC50 was >1000
mg/L (41% inhibition).
The no observed effect concentration (NOEC) for MTF was 30 mg/L
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