Registration Dossier
Registration Dossier
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EC number: 265-078-2 | CAS number: 64741-77-1 A complex combination of hydrocarbons from distillation of the products from a hydrocracking process. It consists predominantly of saturated hydrocarbons having carbon numbers predominantly in the range of C10 through C18, and boiling in the range of approximately 160°C to 320°C (320°F to 608°F).
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: no informtion on GLP. Study report lacks some details but nevertheless provides useful supporting information
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- not specified
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- 68334-30-5
- Cas Number:
- 68334-30-5
- IUPAC Name:
- 68334-30-5
- Reference substance name:
- Diesel Fuel
- IUPAC Name:
- Diesel Fuel
- Test material form:
- other: low viscosity liquid hydrocarbon
- Details on test material:
- Diesel fuel
Constituent 1
Constituent 2
Method
- Target gene:
- L5178Y mouse lymphoma cells
- Test concentrations with justification for top dose:
- 0.125, 0.188, 0.25, 0.375, 0.5 uL/ml - non activation study
0.064, 0.125, 0.188, 0.25, 0.375, 0.5 uL/ml - activation study - Vehicle / solvent:
- acetone
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ACETONE
- Positive controls:
- yes
- Positive control substance:
- other: ethylmethane sulphonate and dimethyl nitrosamine
- Details on test system and experimental conditions:
- L5178Y MOUSE LYMPHOMA CELLS
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- both with and without activation
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Diesel fuel was not mutagenic in the mouse lymphoma assay - Executive summary:
The genotoxic activity of diesel fuel was evaluated in a mouse lymphoma assay using L5178Y cells. Assays both in the presence and absence of a mouse liver mirosome fraction were conducted. Dose levels ranged between 0.064 and 0.5 uL/ML.
Results showed no evidence of of genotoxic activity, either in the presence or absence of metabolic activation. The positive control materials both showed expected results.
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