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EC number: 211-989-5
CAS number: 732-26-3
The 96 h LC50 in carp was determined to be >0.048 mg/L.
There are three studies to address this endpoint, one key and two
In the key study, the acute toxicity of the test material to carp was
investigated in accordance with the standardised guidelines OECD 203, EU
Method C.1 and the Guidance document on aquatic toxicity testing of
difficult substances and mixtures, OECD series on testing and assessment
number 23 under GLP conditions. The study was assigned a reliability
score of 1 in line with the principles for assessing data quality as
defined by Klimisch et al. (1997).
Following an initial range-finding test, a full test was performed with
Cyprinus carpio under semi-static conditions. The test material was not
completely soluble in test medium at the loading rate initially
prepared; therefore a Water Soluble Fraction (WSF) was prepared at a
loading rate of 100 mg/L and used as the highest concentration. The
lower test concentrations were prepared by subsequent dilutions of the
highest concentration in test medium. The final test solutions were all
clear and colourless. Test solutions were prepared daily.
Seven fish per group were exposed to an untreated control and solutions
containing 10, 18, 32, 56 and 100 % WSF. The total exposure period was
96 hours. Test solutions were renewed on a daily basis. Samples for
analytical confirmation of the exposure concentrations were taken at the
start and at the end of the first and the last refreshment period.
The responses recorded in this test allowed for reliable determination
of an LC50. No mortality was observed in any of the groups tested. In
the 100 % WSF, at 96 hours one fish was observed to be immobile and 6
fish were observed to be hypoactive. Samples taken from the highest
concentration were analysed; the average exposure concentration was
calculated and corresponded to 0.048 mg/L.
Under the conditions of this study, the 96 h LC50 exceeded an average
exposure concentration of 0.048 mg/L, being considered the maximum
soluble concentration in test medium.
Both of the supporting studies were assigned a reliability score of 2 in
line with the principles for assessing data quality as defined by
Klimisch et al. (1997).
The first supporting study, the toxicity of the test material to fish
was estimated using the ECOSAR model v1.11 (EPI Suite v4.11).
The structure-activity relationships (SARs) presented in this program
are used to predict the aquatic toxicity of chemicals based on their
similarity of structure to chemicals for which the aquatic toxicity has
been previously measured. Most SAR calculations in the ECOSAR Class
Program are based upon the octanol/water partition coefficient
(Kow). The toxicity of the test material to fish was estimated using the
Phenols class of ECOSAR. The molecular weight of the test material is
within the training set range.
The estimated 96 hour LC50 was 0.021 mg/L.
In the second supporting study, the potential of the test material to
cause acute toxicity to fish was investigated in a study conducted
broadly in line with the standardised guideline OECD 203.
Following a range-finding test, the test material was administered to 10
Rainbow trout (Oncorhynchus mykiss) at a concentration of 0.10 mg/L in 1
% v/v Tween 80 – tetrahydrofuran for a period of 96 hours. Concurrent
negative and solvent controls were each run with 10 fish.
No mortality was seen in either replicate of the treated fish, or in the
control or solvent control.
Under the conditions of this study the LC50 was determined to be > 0.10
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