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EC number: 211-989-5
CAS number: 732-26-3
The 72 h NOEC for both growth rate inhibition and yield inhibition was determined to be >0.040 mg/L.
There are two studies available to address this endpoint, one key and
In the key study, the toxicity of the test material to the freshwater
green alga Pseudokirchneriella subcapitata was investigated in
accordance with the standardised guidelines OECD 201, EU Method C.3 and
the Guidance document on aquatic toxicity testing of difficult
substances and mixtures, OECD series on testing and assessment number 23
under GLP conditions. The study was assigned a reliability score of 1 in
line with the principles for assessing data quality as defined by
Klimisch et al. (1997).
Following a combined limit/range-finding test, a full test was
performed. The test material was not completely soluble in test medium
at the loading rate initially prepared. A Water Soluble Fraction (WSF)
was prepared at a loading rate of 100 mg/L and used as the highest
concentration. The lower test concentrations were prepared by subsequent
dilutions of the highest concentration in test medium. The final test
solutions were all clear and colourless.
Six exponentially growing algal cultures were exposed to an untreated
control, whereas three replicates per group were exposed to solutions
containing 1.0, 3.2, 10, 32 and 100 % WSF. Initial cell density was 10⁴
cells/mL. The total exposure period was 72 hours and samples for
analytical confirmation of exposure concentrations were taken at the
start, after 24 and 72 hours of exposure. As the test material was
potentially volatile, the exposure was performed in airtight closed
vessels, with headspace reduced to minimum and adjusted M2 medium.
Samples taken from all groups were analysed. The actual concentrations
in the two lowest groups were below the limit of detection at the start
of the test. The concentrations measured in the 10, 32 and 100 % WSF
increased with the applied dose. At the end of the test no concentration
could be detected in the 10 % WSF. The concentrations measured in the 32
and 100 % WSF were at the level of 56 and 21 % of initial at the end of
the test, respectively. Based on these results, the Time Weight Average
concentrations calculated for the two highest concentrations were 0.019
and 0.040 mg/L for the 32 and 100 % WSF, respectively.
The EC50 for growth rate inhibition (72 h ErC50) and the EC50 for yield
inhibition (72 h EyC50) was beyond the range tested, i.e. exceeded a TWA
concentration of 0.040 mg/L (considered the maximum soluble in test
Under the conditions of the study, the 72 h NOEC for both growth rate
inhibition and yield inhibition was >0.040 mg/L. Due to the very low
solubility of the test material in water, concentration levels that
might be toxic for algae could not be reached.
In the supporting study, the toxicity of the test material to green
algae was estimated using the ECOSAR model v1.11 (EPI Suite v4.11). The
study was assigned a reliability score of 2 in line with the principles
for assessing data quality as defined by Klimisch et al. (1997).
The structure-activity relationships (SARs) presented in this program
are used to predict the aquatic toxicity of chemicals based on their
similarity of structure to chemicals for which the aquatic toxicity has
been previously measured. Most SAR calculations in the ECOSAR Class
Program are based upon the octanol/water partition coefficient
(Kow). The toxicity of the test material to green algae was estimated
using the Phenols class of ECOSAR. The molecular weight of the test
material is within the training set range.
The estimated 96 hour EC50 was 0.134 mg/L.
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