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EC number: 211-989-5
CAS number: 732-26-3
Under the conditions of this study the test material was determined not to be inherently biodegradable.
Two studies are provided to address this endpoint, one experimental and
one prediction. Both were awarded a reliability score of 2 in accordance
with the criteria set forth by Klimisch et al. (1997). The experimental
result has been selected as the key value.
In the key study the inherent biodegradability of the test material was
investigated in a modified MITI (II) test conducted in accordance with
the standardised guideline OECD 302C.
A mixed population of activated sewage sludge microorganisms was exposed
to the test material for a period of 28 days at a concentration of 30
mg/L. The activated sewage was prepared by sampling 10 different sites
around the UK in accordance with the guideline and the inoculum prepared
for exposure by being fed daily with a 0.1 % synthetic sewage and
maintained on a constant aeration at 25 ± 2 °C. The usage rate was
equivalent to 100 mg dry weight/L.
Oxygen consumption was measured by direct manometer reading. Manometer
volumes were recorded daily.
The test material attained 13 % degradation after 28 days, calculated
from oxygen uptake (% of ThOD).
Under the conditions of this study the test material was determined not
to be inherently biodegradable.
In the supporting study the ready biodegradability of the test material
was estimated using the BIOWIN model v4.10 (EPI Suite v4.11).
BIOWIN, the model within EPISUITE which calculates biodegradation,
estimates the probability of rapid aerobic and anaerobic biodegradation
of an organic compound in the presence of mixed populations of
environmental microorganisms. BIOWIN contains seven separate models:
Biowin1 (linear probability model); Biowin2 (nonlinear probability
model); Biowin3 (expert survey ultimate biodegradation model); Biowin4
(expert survey primary biodegradation model); Biowin5 (MITI linear
model); Biowin6 (MITI nonlinear model); and Biowin7 (anaerobic
Sufficient information on the model training set is available to
evaluate the applicability of the model to this substance. The molecular
weight of the test material is within the training set range.
The test material was predicted to be not readily biodegradable.
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