1,3-Cyclohexanedimethanamine

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12 November 1999 to 10 January 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study conducted according to OECD guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

GLP compliance:

yes (incl. QA statement)

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

- Sampling intervals for the parent/transformation products: 0, 2.4, and 120 hours
- Sampling method: GC
- Sampling intervals/times for pH measurements: various times
- Sampling intervals/times for sterility check: not specified
- Sample storage conditions before analysis: pH 4, 7, 9 at 50.0±0.5°C for five days.

Buffers:

- pH: 4
- Type and final molarity of buffer: (0.05, 0.10, 0.05 mol/l)
- Composition of buffer: Citric acid, sodium hydroxide, hydrochloric acid

- pH: 7
- Type and final molarity of buffer: (0.04, 0.03 mol/l)
- Composition of buffer: disodium hydrogen phosphate, potassium dihydrogen phosphate

- pH: 9
- Type and final molarity of buffer: (0.05, 0.02 mol/l)
- Composition of buffer: disodium tetraborate, hydrochloric acid

Details on test conditions:

TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: stoppered glass flasks
- Sterilisation method: Buffer solutions were filtered through a 0.2 micrometer membrane filter to ensure they were sterile before commencement of the test.
- Measures taken to avoid photolytic effects: sheilded from light
- Measures to exclude oxygen: Solutions were subjected to ultrasonication and degassing with nitrogen to minimise dissolved oxygen content.

TEST MEDIUM
- Volume used/treatment: sample solutions were prepared at nomial concentration of 0.2g/l in the buffer solutions.

Duration:

120 h

pH:

4

Initial conc. measured:

188 mg/L

Duration:

120 h

pH:

9

Initial conc. measured:

176 mg/L

Duration:

120 h

pH:

7

Initial conc. measured:

196 mg/L

Number of replicates:

3

Transformation products:

no

% Recovery:

98

pH:

4

Temp.:

50 °C

Duration:

120 h

% Recovery:

97

pH:

7

Temp.:

50 °C

Duration:

120 h

% Recovery:

100

pH:

9

Temp.:

50 °C

Duration:

120 h

pH:

4

Temp.:

50 °C

DT50:

> 1 yr

pH:

7

Temp.:

50 °C

DT50:

> 1 yr

pH:

9

Temp.:

50 °C

DT50:

> 1 yr

Details on results:

TEST CONDITIONS
- pH, sterility, temperature, and other experimental conditions maintained throughout the study: Yes

Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life of greater than 1 year at 25°C at pH 4, 7, and 9.

Validity criteria fulfilled:

yes

Conclusions:

The estimated half-life at 25°C of 1,3-BAC at pH 4, 7 and 9 is greater than 1 year.

Executive summary:

Assessment of hydrolytic stability was carried out using Method 111 of the OECD Guidelines for Testing of Chemicals, 12 May 1981. Sample solutions were prepared in stoppered glass flasks at a nominal concentration of 0.2 g/l in the three buffer solutions. The solutions were shielded from light whilst maintained at the test temperature. Sample solutions at pH 4, 7 and 9 were maintained at 50.0 ± 0.5°C for a period of 5 days. Less than 10% hydrolysis after 5 days at 50°C is equivalent to a half-life of greater than 1 year at 25°C at pH 4, 7, and 9. Therefore the estimated half-life at 25 ºC of 1,3 -BAC at pH 4, 7 and 9 is greater than 1 year.

Description of key information

Less than 10% hydrolysis was seen after 5 days at 50°C, equivalent to a half-life of greater than 1 year at 25°C at pH 4, 7, and 9.

Key value for chemical safety assessment

Half-life for hydrolysis:

1 yr

at the temperature of:

25 °C

Additional information

Assessment of hydrolytic stability was carried out using Method 111 of the OECD Guidelines for Testing of Chemicals, 12 May 1981. Sample solutions were prepared in stoppered glass flasks at a nominal concentration of 0.2 g/l in the three buffer solutions. The solutions were shielded from light whilst maintained at the test temperature. Sample solutions at pH 4, 7 and 9 were maintained at 50.0 ± 0.5°C for a period of 5 days. Less than 10% hydrolysis after 5 days at 50°C, equivalent to a half-life of greater than 1 year at 25°C at pH 4, 7, and 9.