4,4'-isopropylidenediphenol

Toxicity to other above-ground organisms

Administrative data

Endpoint:

toxicity to other above-ground organisms

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Hormone tests were not specific to the polecat, so the actual levels reported may be different than what is stated in the results.  There was no analytical confirmation ofthe nominal BPA concentrations.  Treatment groups were small.

Data source

Reference

Reference Type:

publication

Title:

In vivo effects of bisphenol A on the polecat (Mustela putorius)

Author:

Nieminen P, Lindstrom-Seppa P, Juntunen M, Asikainen J, Mustonen A, Karonen S, Mussalo-Rauhamaa H, Kukkonen JVK

Year:

2002

Bibliographic source:

Journal of Toxicology and Environmental Health, Part A., 65(13), 933-945

Materials and methods

Principles of method if other than guideline:

Method: other

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-isopropylidenediphenol

EC Number:

201-245-8

EC Name:

4,4'-isopropylidenediphenol

Cas Number:

80-05-7

Molecular formula:

C15H16O2

IUPAC Name:

4-[2-(4-hydroxyphenyl)propan-2-yl]phenol

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

not specified

Test organisms

Test organisms (species):

other: Mustela putorius (polecat)

Study design

Study type:

laboratory study

Limit test:

no

Total exposure duration:

14 d

Test conditions

Reference substance (positive control):

not specified

Results and discussion

Any other information on results incl. tables

No macroscopic effects such as activity, feeding, or locomotion differences were observed in polecats in any of the treatment groups. Body weight was unaffected by BPA exposure when comparing across treatment group and sex, but male polecats were found to have a mean body mass almost twice that of females. For this reason the BMI of males was significantly greater than the BMI of females although there was no significant difference between BMIs of different treatment groups. Absolute and relative liver weights were unaffected by BPA exposure in both males and females with the exception of females in the 250 mg/kg body weight/day group, whose mean and relative liver weight were greater than those in the control group. Testosterone, estradiol, and FSH concentrations were not significantly affected by BPA exposure. T3, T4, and TSH levels were not significantly different with respect to treatment group, although females were found to have significantly higher T3/T4 ratios than males. Cortisol concentrations in females were significantly decreased in the 50 mg BPA/kg body weight/day group compared to females in the control. Plasma leptin concentrations did not vary significantly between control and treatment groups. EROD activity was not significantly affected by BPA exposure. GST levels increased significantly in females exposed to 250 mg BPA/kg body weight/day compared to female controls. UDPGT activity was significantly increased in both males (250 mg/kg bw/day) and females (50 and 250 mg/kg bw/day) compared to the control, when exposed to BPA. There was a significant correlation between both UDPGT and GST levels, and BPA dose. Discriminant analysis indicated an overall deviation from the physiology of the control group when polecats were given BPA doses of 50 and 250 mg/kg bw/day with slight changes beginning somewhere between 10 and 50 mg/kg bw/day. The endocrine effects were less pronounced than the change in enzyme activities. BPA induced phase II enzyme reactions (UDPGT and GST) in a dose-dependent manner.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified