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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
Experimental data of test chemicals
Justification for type of information:
Data is from handbook or collection of data
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report was prepared based on the two toxicity study of microorganism for the test chemical.
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: WoE 2. domestic activated sludge, WoE 3. Pseudomonas putida
Details on inoculum:
WoE 2: The activated sludge collected from a municipal sewage treatment plant
Test type:
static
Water media type:
freshwater
Total exposure duration:
3 h
Remarks on exposure duration:
16 hours exposure was provided in WoE 3
Reference substance (positive control):
not specified
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: WoE 2
Duration:
16 min
Dose descriptor:
EC0
Effect conc.:
14.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: WoE 3
Validity criteria fulfilled:
not specified
Conclusions:
The toxicity study were carried out on the activated sludge and Pseudomonas putida. The effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L and based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.
Executive summary:

Studies were reviewed and summarized based on the various results from different sources for the determination of the effects of test chemical on the growth rate inhibition of microorganisms. The studies are as mentioned below:

 

Principle of this study was to determine the effect of test chemical on the domestic activated sludge. Test conducted for 3 hours. The activated sludge collected from a municipal sewage treatment plant. The effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L based on effect observations on population growth rate. Thus, based on the EC50 value, test chemical considers to be toxic.

 

Above study further supported by the weight of evidence study from handbooks. Principle of this study was to determine the effect of test chemical on the population growth rate inhibition of microorganisms. Test conducted for the 16 hours. Based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

 

Based on the above effects on the activated sludge and Pseudomonas putida, the effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L and based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

Description of key information

The toxicity study were carried out on the activated sludge and Pseudomonas putida. The effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L and based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:
100 mg/L

Additional information

Studies were reviewed and summarized based on the various results from different sources for the determination of the effects of test chemical and structurally and functionally similar read across chemicals on the growth rate inhibition of microorganisms. The studies are as mentioned below:

 

Principle of this study was to determine the effect of test chemical on the domestic activated sludge. Test conducted for 3 hours. The activated sludge collected from a municipal sewage treatment plant. The effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L based on effect observations on population growth rate. Thus, based on the EC50 value, test chemical considers to be toxic.

 

Above study further supported by the weight of evidence study from handbooks. Principle of this study was to determine the effect of test chemical on the population growth rate inhibition of microorganisms. Test conducted for the 16 hours. Based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

 

Based on the above effects on the activated sludge and Pseudomonas putida, the effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L and based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.