N,N-dimethyl-p-toluidine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of test chemicals

Justification for type of information:

Data is from handbook or collection of data

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report was prepared based on the two toxicity study of microorganism for the test chemical.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: WoE 2. domestic activated sludge, WoE 3. Pseudomonas putida

Details on inoculum:

WoE 2: The activated sludge collected from a municipal sewage treatment plant

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Remarks on exposure duration:

16 hours exposure was provided in WoE 3

Reference substance (positive control):

not specified

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: WoE 2

Duration:

16 min

Dose descriptor:

EC0

Effect conc.:

14.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: WoE 3

Validity criteria fulfilled:

not specified

Conclusions:

The toxicity study were carried out on the activated sludge and Pseudomonas putida. The effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L and based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

Executive summary:

Studies were reviewed and summarized based on the various results from different sources for the determination of the effects of test chemical on the growth rate inhibition of microorganisms. The studies are as mentioned below:

 

Principle of this study was to determine the effect of test chemical on the domestic activated sludge. Test conducted for 3 hours. The activated sludge collected from a municipal sewage treatment plant. The effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L based on effect observations on population growth rate. Thus, based on the EC50 value, test chemical considers to be toxic.

 

Above study further supported by the weight of evidence study from handbooks. Principle of this study was to determine the effect of test chemical on the population growth rate inhibition of microorganisms. Test conducted for the 16 hours. Based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

 

Based on the above effects on the activated sludge and Pseudomonas putida, the effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L and based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

Description of key information

The toxicity study were carried out on the activated sludge and Pseudomonas putida. The effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L and based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

Additional information

Studies were reviewed and summarized based on the various results from different sources for the determination of the effects of test chemical and structurally and functionally similar read across chemicals on the growth rate inhibition of microorganisms. The studies are as mentioned below:

 

Principle of this study was to determine the effect of test chemical on the domestic activated sludge. Test conducted for 3 hours. The activated sludge collected from a municipal sewage treatment plant. The effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L based on effect observations on population growth rate. Thus, based on the EC50 value, test chemical considers to be toxic.

 

Above study further supported by the weight of evidence study from handbooks. Principle of this study was to determine the effect of test chemical on the population growth rate inhibition of microorganisms. Test conducted for the 16 hours. Based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.

 

Based on the above effects on the activated sludge and Pseudomonas putida, the effective concentration (EC50) value for test material on domestic activated sludge in a 3 hours study were determined to be 100 mg/L and based on the growth rate inhibition of Pseudomonas putida due to the test chemical exposure for 16 hours, the EC0 value was determined to be 29 mg/l.