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EC number: 200-854-6
CAS number: 75-25-2
The dissolved oxygen concentrations after 30
minutes contact time in all vessels were above 60% of the dissolved
oxygen saturation level of 8.9 mg O2/L.
Statistically significant toxic effects were
shown at all the test concentrations employed.
Based on these results it was considered
unnecessary to perform a definitive test.
The results for inhibition of respiration
are presented in the following table:
* Experimental work indicated that the test
item was volatile in solution as when a steady stream of compressed air
(0.5 litres per minute) was passed with a solution of the test item over
three hours, a 88% loss of test item occurred. This was determined by
Total Organic Carbon analysis. Therefore the
3 -hour ECx values and NOEC for the test
item may be underestimates.
study performed to assess the effect of the test substance on the
respiration of activated sewage sludge. The method followed was designed
to be compatible with the OECD Guidelines for Testing of Chemicals
(2010) No. 209 "Activated Sludge, Respiration Inhibition Test (Carbon
and Ammonium Oxidation)".
sewage sludge was exposed to an aqueous solution of the test item at
concentrations of 10, 100 and 1000 mg/L (three replicates of the 1000
mg/L test concentration) for a period of 3 hours at a measured
temperature of between 20 °C and 21 °C with the addition of a synthetic
sewage as a respiratory substrate.
The rate of respiration was
determined after 3 hours contact time and compared to data for the
control and a reference item, 3,5-Dichlorophenol.
test substance gave a No
Observed Effect Concentration (NOEC) after 3 hours exposure was less
than 10 mg/L. However, analysis
during the test indicated that the test item was volatile in solution as
when a steady stream of compressed air (0.5 litres per minute) was
passed with a solution of the test item over three hours, a 88% loss of
test item occurred. Therefore the 3-Hour EC50
for the test item is considered to be underestimate and not relevant for
use in assessment of actual toxicity to sludge microorganisms in
reference item gave a 3-Hour EC50
value of 8.2 mg/L, 95%
confidence limits 6.4 to 11 mg/L.
Toxicity of Bromoform (EC 200 -854 -6) to
sewage sludge microorganisms.
The test substance gave a 3-hour EC50 > 1000
mg/L and a 3 -hour No Observed Effect Concentration (NOEC) of < 10 10
Analysis during the test indicated that the
test item was volatile in solution as when a steady stream of compressed
air (0.5 litres per minute) was bubbled through a solution of the test
item over three hours, a 88% loss of test item occurred. Therefore the
3-Hour EC50 and NOEC value for the test substance are considered to be
underestimated as a significant proportion of the test substance
vapourised from the test medium. As rapid volatisation and loss of the
test substance can be expected to also occur in a waste water treatment
plant, the 3-hour EC50 and NOEC values are not considered relevant for
use in assessment of actual toxicity to sludge microorganisms, as little
or no test substance is expected to be present in the waters within the
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