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EC number: 200-854-6 | CAS number: 75-25-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Sediment toxicity
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
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- Specific investigations
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- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental Phase: 01 May 2018 to 29 May 2018. Report Issued: 01 October 2018.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- One deviation occured but it was considered to have had no adverse effect on the integrity of the study given that all validation criteria were met for the study.
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- A mixed population of sewage treatment micro-organisms was obtained on 01 May 2018 from the final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.
The sample of effluent was filtered through coarse filter paper (first approximate 200 mL discarded) and the filtrate maintained on continuous aeration in a temperature controlled room at approximately 21°C prior to use. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 33 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Acitivated Sludge
- Test temperature: 21°C
- pH: 7.4 - 7.5 (at start on the test)
- pH adjusted: yes
- Aeration of dilution water: No
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: 250 mL BOD bottles (darkened glass) with ground glass stoppers.
- Number of culture flasks/concentration: Sufficient bottles were prepared to allow a single oxygen determination per bottle with duplicate bottles for each test preparation at each sampling occasion.
- Measuring equipment: YSI 54A dissolved oxygen meter and BOD Probe
- Test performed in open system: No bottles were firmly stoppered.
MEDIUM
- Mineral Medium used: The mineral medium used in the study was that recommended in the OECD Guideline.
SAMPLING
- Sampling frequency: Dissolved oxygen concentrations in duplicate samples from the control, reference and test item series were determined, on Days 0, 3, 5, 7, 10, 14, 21 and 28 by means of a YSI 54A. Dissolved oxygen meter and BOD Probe. Dissolved oxygen concentrations in duplicate samples from the toxicity control series were determined on Days 0, 14 and 28 only.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: Yes - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Concentration used: 3.0 mg/L
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 6
- Sampling time:
- 28 d
- Results with reference substance:
- The reference item (sodium benzoate), attained 64% biodegradation after 14 days with greater than 60% degradation being attained in a 10-Day window. After 28 days 71% biodegradation was attained. These results confirmed the suitability of the inoculum and test conditions and satisfied the validation criterion given in the OECD Test Guidelines.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item attained 6% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the terms and conditions of OECD Guideline No 301D.
- Executive summary:
Introduction
A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301D, "Ready Biodegradability; Closed Bottle Test”
Methods
The test item, at a concentration of 33 mg/L, was exposed to sewage treatment micro-organisms with mineral medium in sealed culture vessels in the dark at approximately 21°C for 28 days. The degradation of the test item was assessed by the determination of the amount of oxygen consumed. Control solutions with inoculum and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.
Result and Conclusion
The test item attained 6% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the terms and conditions of OECD Guideline No 301D.
Reference
Description of key information
Ready biodegradability of the Test Substance.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
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