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EC number: 200-854-6 | CAS number: 75-25-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Specific investigations
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- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Acute toxicity of the test substance.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Although no guideline is mentioned in the publication the method follows the classical LD50 test.
- GLP compliance:
- no
- Test type:
- other: Although no guideline is mentioned in the publication the method follows the classical LD50 test
- Limit test:
- no
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Aldrich Chemiclas, Milwaukee, Wis.
- Purity: 96%
- Impurity: 3% chlorodibromomethane, 1% not stated. - Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Biobreeding Labs., Ottawa, Ontario.
- Females (if applicable) nulliparous and non-pregnant: Not stated.
- Age at study initiation: Not stated
- Weight at study initiation: 150-200g (start of acclimation)
- Fasting period before study: Fasted overnight before dosing
- Housing: Not stated.
- Diet (e.g. ad libitum): Not stated.
- Water (e.g. ad libitum): Not stated.
- Acclimation period: One week.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 ± 2 °C
- Humidity (%): 40 - 60%
- Air changes (per hr): Not stated.
- Photoperiod (hrs dark / hrs light): Not stated. - Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: Not specified
- Amount of vehicle (if gavage): 2 ml/kg
MAXIMUM DOSE VOLUME APPLIED: 2 ml/kg
- Doses:
- Range-finding:
To establish the dose levels to be used in the LD5O studies, groups of two animals were administered test compounds at 250-3000 mg/kg and the mortality data were recorded.
Main study doses:
546 mg/kg
765 mg/kg
1071 mg/kg
1500 mg/kg
2100 mg/kg - No. of animals per sex per dose:
- 10 males and 10 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs - Statistics:
- Median lethal dose (LD50) was calculated using the probit analysis.
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 388 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 167 - <= 1 693
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 147 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 890 - <= 1 524
- Mortality:
- Number of dead animals per group:
546 mg/kg = 1/20
765 mg/kg = 5/20
1071 mg/kg = 5/20
1500 mg/kg = 9/20
2100 mg/kg = 20/20 - Clinical signs:
- other: Hypothermia was observed, but dose levels not stated.
- Gross pathology:
- Livers and kidneys were reported to be congested and enlarged at necropsy, but no dose response information was reported.
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The acute oral LD50 of the test substance in Sprague-Dawley rats was 1388 and 1147 mg/kg bw in males and females respectively.
- Executive summary:
Introduction
The acute oral toxicity of the test substance to Sprague-Daawley rats. No guideline was followed but the study used a method similar to the classical LD50 test.
Method
To establish the dose levels to be used groups of two animals were administered test compounds at 250-3000 mg/kg and the mortality data were recorded and used ot det dosew in the main test. For the main test five doses between 546 and 2100 mg/kg were employed and 10 males and 10 females used at each dose level. Dosing solutions were administered to animals in corn oil using premature infant feeding tubes (8 Fr., 15 in. length) at a maximum volume of 2ml/kg. Clinical observations were made for 14 days after dosing and a post-mortem examination was performed on animals which died during this period. All surviving animals were killed at the end of 14 days and subjected to gross pathologic examination.
Results and Conclusion
The acute oral LD50 of the test substance in Sprague-Dawley rats was 1388 and 1147 mg/kg bw in males and females respectively.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 147 mg/kg bw
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for classification or non-classification
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