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EC number: 200-854-6 | CAS number: 75-25-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: The acute toxicity tests followed procedures presented in "Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians" (U.S. EPA 1975).
- GLP compliance:
- no
- Specific details on test material used for the study:
- All chemicals (the study was part of a series on multiple chemicals) tested during the study were >80% pure.
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- TEST ORGANISM
- Common name: Blue Gill
- Source: Test animals utilised were young of the year bluegill (L. macrochirus) obtained from commercial fish suppliers within the continental United States.
- Age at study initiation (mean and range, SD): not given
- Weight at study initiation (mean and range, SD): 0.32 - 1.2g
ACCLIMATION
- Acclimation period: not stated
- Acclimation conditions (same as test or not): No
- Acclimiation water: Well water at a minimum of four volume replacements per day. Chemical and physical characteristics of this well-water were measured weekly, revealing a total hardness of 28-44 mg/L CaC03, a total alkalinity of 20-30 mg/L CaCO3, a pH of 6.7-7.4, a dissolved oxygen concentration of 5.3-7.0 mg/L, a specific conductance of 95-170 µmhos/cm and a temperature of 20-24 °C
FEEDING DURING TEST
- Food type: Daily dry, pelleted food and once weekly with raw ground beef liver. All feeding was discontinued for 48 h prior to testing.
- Frequency: ad libitum - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 32-48 mg/L CaCO3 (dilution water)
- Test temperature:
- 21 -23 °C
- pH:
- 7.9 - 6.5
- Dissolved oxygen:
- 9.7 mg/l at the start of exposure to 0.3 mg/l at the end of the test.
- Conductivity:
- 3-190 umhos/cm (dilution water)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 19.6 litre widemouthed glass jars
- Test vessel fill volume: 15 litres of test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): not specified (test substance identifed as soluble and it is not clear from the paper if a solvent/ vehicle was used)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water used to prepare the test solutions was deionised water reconstituted according to recommended procedures (U.S. EPA 1975). The water had a total hardness of 32-48 mg/L CaCO3, a total alkalinity of 28-34 mg/L CaC03, a pH of 6.7-7.8, a dissolved oxygen concentration of 7.0-8.8 mg/L,
a specific conductance as 93-190 µmhos/cm.
OTHER TEST CONDITIONS
- Adjustment of pH: Not stated
- Photoperiod: Not stated
- Light intensity: Not stated.
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 29 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Reported statistics and error estimates:
- 96-hour LC50 95% confidence limits were 24 -36 mg/L.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96-hour LC50 of the test substance to Bluegill (Lepomis macrochirus) was 29 mg/l with 95% confidence limits of 24 -36 mg/L.
- Executive summary:
Introduction
The toxicity of the test substance to the freshwater fish BlueGill (Lepomis macrochirus) was investigated using procedures presented in "Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians" (U.S. EPA 1975).
Method
Ten fish were randomly selected from a test population and added to each test jar within 30 min after the addition of the test chemical. est substance free controls were also maintained and capped where appropriate. Controls were maintained under the same conditions as the test solutions. Fish were exposed for a total of 96 hours. Fish mortality, behavioural and physiological responses, and solution appearance were observed and recorded at time 0 and every 24 h during exposure.
Results and Conclusion
The 96-hour LC50 of the test substance to Bluegill (Lepomis macrochirus) was 29 mg/l with 95% confidence limits of 24 -36 mg/L.
Reference
Description of key information
Acute toxicity of the test substance to fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 29 mg/L
Additional information
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