Dinitrotoluene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without analytical dose verification, study was peer reviewed in OECD SIDS (2004)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Reference 2

Reference Type:

publication

Title:

QSAR study of the toxicity of nitrobenzene derivatives towards Daphnia magna, Chlorella pyrenoidosa and Photobacterium phosphoreum.

Author:

Deneer JW, van Leeuwen CJ, Seinen W, Maas-Diepeveen JL, Hermens JLM

Year:

1989

Bibliographic source:

Aquatic Toxicol. 15, 83-98. As cited in: OECD SIDS, Dinitrotoluene (isomers mixture), CAS No. 25321-14-6, 2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

1984

Deviations:

yes

Remarks:

modifications according to van Leeuwen et al. (1985)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,3-dinitrotoluene

EC Number:

210-013-5

EC Name:

2,3-dinitrotoluene

Cas Number:

602-01-7

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-2,3-dinitrobenzene

Constituent 2

Reference substance name:

2,4-dinitrotoluene

EC Number:

204-450-0

EC Name:

2,4-dinitrotoluene

Cas Number:

121-14-2

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-2,4-dinitrobenzene

Constituent 3

Reference substance name:

2,6-dinitrotoluene

EC Number:

210-106-0

EC Name:

2,6-dinitrotoluene

Cas Number:

606-20-2

Molecular formula:

C7H6N2O4

IUPAC Name:

2-methyl-1,3-dinitrobenzene

Constituent 4

Reference substance name:

3,4-dinitrotoluene

EC Number:

210-222-1

EC Name:

3,4-dinitrotoluene

Cas Number:

610-39-9

Molecular formula:

C7H6N2O4

IUPAC Name:

4-methyl-1,2-dinitrobenzene

Details on test material:

- Name of test material (as cited in study report):
2,3-Dinitrotoluol (2,3-DNT), CAS No. 602-01-7
2,4-Dinitrotoluol (2,4-DNT), CAS No. 121-14-2
2,6-Dinitrotoluol (2,6-DNT), CAS No. 606-20-2
3,4-Dinitrotoluol (3,4-DNT), CAS No. 610-39-9
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Chlorella pyrenoidosa

Details on test organisms:

TEST ORGANISM
- Common name: Unicellular alga Chlorella pyrenoidosa
- Strain: Wisconsin 2005
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, Cambridge

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Details on test conditions:

TEST SYSTEM
- Test vessel: 200 ml Erlenmeyer flask
- incubated on mechanical shaker
- Initial cells density: 10E+8 cells/L (100 mL)

GROWTH MEDIUM
- Algal medium according to van Leeuwen and Maas (1985)

OTHER TEST CONDITIONS
- Light intensity and quality: fluorescent light (7.5 W/m2)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Cells were counted with ZBI Coulter Counter using 70 μm aperture

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

0.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,3-DNT

Basis for effect:

growth rate

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

0.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,4-DNT

Basis for effect:

growth rate

Effect concentrations 3

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

6.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,6-DNT

Basis for effect:

growth rate

Effect concentrations 4

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

0.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

3,4-DNT

Basis for effect:

growth rate

Any other information on results incl. tables

Population growth:

	log EC50 (µM)	EC50 (mg/L)
2,3 -DNT	0.70	0.9
2,4 -DNT	0.70	0.9
2,6 -DNT	1.57	6.7
3,4 -DNT	0.61	0.7