Dinitrotoluene

Biodegradation in soil

Administrative data

Endpoint:

biodegradation in soil

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles. Basic data given.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Materials and methods

Test guideline

Guideline:

other: BBA Nr. IV-4-1 part 2 adopted Dec. 1986 "Biodegradation in soil"

GLP compliance:

yes

Test type:

laboratory

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dinitrotoluene

EC Number:

204-450-0

EC Name:

2,4-dinitrotoluene

Cas Number:

121-14-2

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-2,4-dinitrobenzene

Details on test material:

- Name of test material (as cited in study report): 2,4-Dinitrotoluene
- Analytical purity: > 99%

Radiolabelling:

no

Study design

Oxygen conditions:

aerobic

Soil classification:

other: LUFA St. 2.2 (loamy sand)

Soil properties

Soil no.:

#1

Soil type:

loamy sand

% Clay:

5.1

% Silt:

13

% Sand:

82

% Org. C:

2.3

pH:

> 5.8 - < 6.3

CEC:

9.7 meq/100 g soil d.w.

Duration of test (contact time)

Soil No.:

#1

Duration:

100 d

Initial test substance concentration

Soil No.:

#1

Initial conc.:

5 mg/kg soil d.w.

Parameter followed for biodegradation estimation:

test mat. analysis

Experimental conditions

Soil No.:

#1

Temp.:

20-22 °C

Microbial biomass:

48 mg biomass/100 g soil dry weight

Results and discussion

% Degradation

Key result

Soil No.:

#1

% Degr.:

50

Parameter:

test mat. analysis

Sampling time:

7 d

Half-life / dissipation time of parent compound

Key result

Soil No.:

#1

DT50:

7 d

Type:

(pseudo-)first order (= half-life)

Remarks on result:

other: DT90: = 191.4 day(s) mathematically extrapolated

Transformation products:

not specified

Any other information on results incl. tables

- Microbial biomass: 48.0-51.6 mg/100 g at day 0; 24.0-30.8 mg/100 g soil at the end of the test.
- No differentiation is possible whether abiotic or biotic degradation has occurred, since no sterile control was performed.

- As the DT90 value (191.4 d) is outside the range of the test period (100 d), this value can only be regarded as an
order of magnitude.