Registration Dossier
Registration Dossier
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EC number: 246-836-1 | CAS number: 25321-14-6
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with national standard methods (as cited in OCED SIDS, Dinitrotoluene (isomer mixture), CAS No. 25321-14-6, 2004). Relevant information on the tested substance (e.g. purity) and on test concentration is missing.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Japanese Industrial Standard (JIS K 0102-1986-71) "Testing methods for industrial wastewater
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Dinitrotoluene
- EC Number:
- 246-836-1
- EC Name:
- Dinitrotoluene
- Cas Number:
- 25321-14-6
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- Reaction mass of 1-methyl-2,4-dinitrobenzene and 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 2,4/2,6-Dinitrotoluene (technical mixture)
- Analytical purity: no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Orange-red killifish
- Source: Nakashima fish farm, Daimyojin Nagasu-cho Tamana-gun Kumamot 969-01 Japan
- after external desinfection, the fish were reared in a flow-through system for 3-5 weeks
- Water temperature, pH, dissolved oxygen were continuously measured
- Total hardness, COD, chloride and other parameters were measured exery 6 months
ACCLIMATION
- fish were reared in an acclimatization tank for 28 d at 24°C
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 25+/-2°C
- Nominal and measured concentrations:
- no data
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 4 L round glass vessels
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Water was groundwater from the Kurume Research Laboratories
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 27 mg/L
- Nominal / measured:
- nominal
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
