3-aminopropyldiethylamine

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02. May 1961 - 19. May 1961

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1961

Report date:

1961

Materials and methods

Principles of method if other than guideline:

Two animals were treated for 1, 5, 15 min using occlusive conditions. An application site of 2.5x2.5 cm was covered with the liquid test substance. After the application time (1, 5, 15 min) the skin was washed with Lutrol (conc.) and Lutrol/water (1:1). The animals were observed for 8 days and skin changes were recorded daily. The report describes findings after 24 hours and at the end of the observation period (8 days). For a final evaluation, the findings after 48 and 72 hours from the raw data were taken into account.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-aminopropyldiethylamine

EC Number:

203-236-4

EC Name:

3-aminopropyldiethylamine

Cas Number:

104-78-9

Molecular formula:

C7H18N2

IUPAC Name:

(3-aminopropyl)diethylamine

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Diaethylaminopropylamin

Test animals

Species:

rabbit

Strain:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.02 and 2.31 kg

Test system

Type of coverage:

occlusive

Preparation of test site:

other: intact skin

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Duration of treatment / exposure:

1 min, 5 min and 15 min

Observation period:

14 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: 2.5x2.5 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): The skin was washed with Lutrol (conc.) and Lutrol/water (1:1)
- Time after start of exposure: 1, 5 or 15 min

Results and discussion

In vivo

Results

Irritation parameter:

other: Necrosis

Time point:

other: 24 h to 8 day

Reversibility:

not reversible

Remarks on result:

other: Exposure time: 1, 5 or 15 min

Any other information on results incl. tables

The application of the test substance caused necrosis to the exposes tissues and therefore a precise evaluation of erythema and edema scores was not possible.

Major findings after 1, 5 or 15 min exposure: After 24 h leather-like brown-reddish necrosis with partly reddish partly anaemic surroundings was observed. 8 days post application hard, almost black necrosis was observed.

Applicant's summary and conclusion

Interpretation of results:

Category 1A (corrosive)

Executive summary:

Two animals were treated for 1, 5, 15 min using occlusive conditions with 3-aminopropyldiethylamine. An application site of 2.5x2.5 cm was covered with the liquid test substance. After the application time (1, 5, 15 min) the skin was washed with Lutrol (conc.) and Lutrol/water (1:1). The animals were observed for 8 days and skin changes were recorded daily. The report describes findings after 24 hours and at the end of the observation period (8 days). For a final evaluation, the findings after 48 and 72 hours from the raw data were taken into account. Twenty-four hours after the 1, 5 or 15-min exposure, leather-like brown-reddish necrosis with partly reddish partly anaemic surroundings was observed. Eight days post application hard, almost black necrosis was observed.