3-aminopropyldiethylamine

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03. May 1961 - 12. May 1961

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1961

Report date:

1961

Materials and methods

Principles of method if other than guideline:

50 µL of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after 10 min, 1 and 3h on the day of treatment and up to 8 days afterwards. Findings were recorded daily. The eyes were not washed out after 24 hours as specified in OECD Guideline 405. The report describes findings after 1 and 24 hours and at the end of the observation period. For final evaluation, the findings after 48 and 72 hours from the raw data were taken into account.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-aminopropyldiethylamine

EC Number:

203-236-4

EC Name:

3-aminopropyldiethylamine

Cas Number:

104-78-9

Molecular formula:

C7H18N2

IUPAC Name:

(3-aminopropyl)diethylamine

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Diaethylaminopropylamin

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.01 and 3.23 kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: The adjacent eye served as control treated with 0.9 % NaCl (saline).

Duration of treatment / exposure:

single application

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Results and discussion

In vivo

Results

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: 24 h - 48 h - 72 h

Score:

4

Max. score:

4

Reversibility:

not reversible

Remarks:

corrosion/eye leakage

Remarks on result:

other: necrosis

Other effects:

Gross pathology: 1 animal with invagination of the eye and 1 animal with phanophtalmia.

Any other information on results incl. tables

The application of the test substance caused necrosis and suppuration to the eyes and therefor a precise evaluation of edema and iritis scores was not possible.

Shortly after exposure (10 min) animals showed bloody eye discharge and corrosion was noted. After 2 days, swelling of the eyelids was observed. 5 days post application vessel ingrow (animal1) and suppuration (animal2) was noted. Animal 1 was sacrificed ahead of schedule on day 5 (complete leakage of eye).

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye)

Executive summary:

Fifty µL of 3-aminopropyldiethylamine were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after 10 min, 1 and 3h on the day of treatment and up to 8 days afterwards. Findings were recorded daily. The eyes were not washed out after 24 hours.The application of the test substance caused necrosis and suppuration to the eyes and therefore a precise evaluation of edema and iritis scores was not possible. Shortly after exposure (10 min) animals showed bloody eye discharge and corrosion was noted. After 2 days, swelling of the eyelids was observed. Five days post application vessel ingrow (animal 1) and suppuration (animal 2) was noted. Animal 1 was sacrificed ahead of schedule on day 5 (complete leakage of eye).