3-aminopropyldiethylamine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 May 1961 - 15 Jun 1961

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1961

Report date:

1961

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-aminopropyldiethylamine

EC Number:

203-236-4

EC Name:

3-aminopropyldiethylamine

Cas Number:

104-78-9

Molecular formula:

C7H18N2

IUPAC Name:

(3-aminopropyl)diethylamine

Details on test material:

- Name of test material (as cited in study report): Diaethylaminopropylamin

Test animals

Species:

rat

Strain:

other: Meyer-Arend rats

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 153 g (mean)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

200, 400, 800, 1000, 1250 or 1600 cmm/kg bw (166, 322, 664, 830, 1037.5, 1328 mg/kg bw - conversion in mg/kg is based on density: d=0.83 g/cm3 (BASF AG MSDS)

No. of animals per sex per dose:

166, 322, 644 mg/kg: 2 males, 3 females.
830 mg/kg: 4 females, 6 males.
1037.5 mg/kg: 4 males, 6 females.
1328 mg/kg: 2 males, 3 females.

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 830 mg/kg bw

Mortality:

1328 mg/kg: all animals died within 24 h.
1037.5 mg/kg: 4 females died within 24 h and 1 male within 48 h.
830 mg/kg: 3 males and 1 female died within 24 h. 1 male died within 3 days and 1 male within 6 days.
644 mg/kg: 1 male died on day 7.

Clinical signs:

other: 1328 mg/kg: staggering, beginning lameness of hind limbs, apathy, abdominal position, accelerated respiration. 1037.5 mg/kg: apathy, ruffled fur. 870 mg/kg: ruffled fur, animals were calmer as usual. 644 mg/kg: calmer as usual.

Gross pathology:

1328 mg/kg: 3x stomach filled with red-yellowish content.
1037.5 mg/kg: 1x stomach filled with red-yellowish content, congested liver.
830 mg/kg: 3x stomach filled with red-yellowish content, 2x congestion of liver, spleen and kidneys. 1x small intestine reddened, diarrhoea. 1x fundus reddened.
644 mg/kg: congestion of the lung, distinct peritonitis.

Sacrificed animals:
644 mg/kg: 1x slight paleness of kidney.

Any other information on results incl. tables

Mortality:

Dose (mg/kg bw)	conc. (%)	1 h	24 h	48 h	day 7	day 14
1328	20	0/5	5/5	5/5	5/5	5/5
1037.5	10	0/10	4/10	5/10	5/10	5/10
830	10	0/10	4/10	4/10	6/10	6/10
644	8	0/5	0/5	0/10	1/10	1/10
322	8	0/5	0/5	0/10	0/10	0/10
166	2	0/5	0/5	0/10	0/10	0/10

There is indication that the test substance causes local irritation to exposed tissues.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category IV

Executive summary:

In a study comparable to the OECD Guideline 401, test groups of 5 or 10 rats were treated by single gavage application with an aqueous solution of 3 -aminopropyldiethylamine. The animals were observed for mortality and for clinical symptoms of toxicity. They were weighed prior treatment and thereafter, day 3, day 7 and day 13 post-treatment. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy. The LD50 value was estimated to be ca. 830 mg/kg on the basis of the observed mortalities.