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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1967
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
unsuitable test system

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1967
Report date:
1967

Materials and methods

Principles of method if other than guideline:
The test demonstrates the toxicity of an atmosphere enriched with the volatile parts of the test substance at 20 °C. Young adult laboratory rats, 3-6 per sex, were exposed sequentially to the vapors generated by blowing 200 L/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 30 minutes or 1 or 3 hours. The exposure was subsequently repeated in the same manner. No analytical determination of the atmosphere concentrations was performed. The nominal concentration was calculated as quotient of the amount of test substance weight loss during exposure, and the amount of air used during exposure. Group-wise documentation of clinical signs was performed over a 7 day study period. Body weight of groups was determined before the start of the study and at the end of the observation period. Gross necropsy of the animals was performed after death of the animals or at the end of the study.
GLP compliance:
no
Test type:
other: Inhalation hazard test

Test material

Constituent 1
Chemical structure
Reference substance name:
α,α'-propylenedinitrilodi-o-cresol
EC Number:
202-374-2
EC Name:
α,α'-propylenedinitrilodi-o-cresol
Cas Number:
94-91-7
Molecular formula:
C17H18N2O2
IUPAC Name:
α,α'-propylenedinitrilodi-o-cresol
Details on test material:
- Name of test material (as cited in study report): Keromet MD
- Physical state: liquid
- Analytical purity: ca. 80 %
- Impurities (identity and concentrations): toluene, ca. 20 %

Test animals

Species:
rat
Strain:
other: US-rats
Sex:
male/female
Details on test animals or test system and environmental conditions:
no data

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: toluene
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
>= 30 - <= 180 min
Concentrations:
30 min exposure: ca. 52 mg/l (probably mostly toluene)
60 min exposure: ca. 41 mg/l (probably mostly toluene)
180 min exposure: ca.16 mg/l (probably mostly toluene)
No. of animals per sex per dose:
3 males and 3 females for the 30 and 60 mins of exposure
6 males and 6 females for the 180 mins of exposure
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology
Statistics:
no data

Results and discussion

Mortality:
0/12 animals died after 30 mins of exposure, 1/6 animals died after 60 mins of exposure, 2/6 animals died after 180 mins of exposure
Clinical signs:
other: attempts to escape, irritation of mucous membranes, narkosis due to toluene solvent
Body weight:
no data
Gross pathology:
some animals with chronic bronchitis, otherwise organs without findings

Applicant's summary and conclusion

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