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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage
- Concentration of dry substance 30 mg/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
aniline
Key result
Parameter:
% degradation (O2 consumption)
Value:
>= 70 - <= 80
Sampling time:
28 d
Details on results:
- Duration of adaptation phase (d): 2
- Duration of degradation phase (d): 18
- Degradation at end of ten-day window (% BOD/ThOD): 60 - 70
- Degree of degradation of test substance in abiotic control at end of test (%BOD/ThOD): 0 - 10

Kinetic of test substance (in %):
= 0 after 1 day(s)
= 16 after 2 day(s)
= 52 after 3 day(s)
= 64 after 14 day(s)
= 68 after 21 day(s)
= 75 after 28 day(s)

Kinetic of control substance (in %):
= 60 ... 70 after 14 day(s)
Key result
Parameter:
BOD5*100/ThOD
Value:
60 - 70
Results with reference substance:
- Degradation degree of reference substance after 14 days (%BOD/ThOD): 60 - 70
- Degradation degree in inhibition control after 14 days (%BOD/ThOD): 60 - 70
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The biodegradation rate was determined by measuring the oxygen consumption of non-adapted domestic activated sludge at a concentration of 100 mg/l. After the 28 day incubation period, a degradation rate of 70 -80% was observed.

Description of key information

The ready biodegradability of the substance was tested in a GLP guideline study according to OECD 301F (Ready biodegradability: Manometric Respirometry Test).

The biodegradation rate was determined by measuring the oxygen consumption of non-adapted domestic activated sludge at a concentration of 100 mg/l. After the 28 day incubation period, a degradation rate of 70 -80% was observed.

The substance is therefore considered to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

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