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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
- 40 mg of the test item was weighed into a glass flask and quantitatively transferred into a volumetric flask by multiple washing with the test medium in a total volume of 1000 mL.
- After a thorough mixing the stock mixture was not homogeneous with visible not dissolved parts of the test item.
- Therefore, the stock mixture in a tightly closed flask was sonicated with heating at 40°C for 20 min and then mixed by mechanic shaker at room temperature for 72 h.
- The homogeneous and transparent stock solution of yellow colour and characteristic odour was used as the highest test concentration and was used to prepare the lower test concentrations by sequential dilution with the test medium.
- The solutions of the test item were homogeneous, transparent of yellow colour and characteristic odour and no changes in appearance were recorded during the test.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Institute of Industrial Organic Chemistry Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology
- Age at study initiation (mean and range, SD): < 24 h old at test initiation
- Feeding during test: no
- Food type: Pseudokirchneriella subcapitata algae suspension
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20.8 – 22.7 ºC
pH:
Fresh solution at exposure initiation: 7.60 - 8.15
Old solutions at renewal: 7.70 - 8.11
Dissolved oxygen:
Fresh solution at exposure initiation: 7.07 - 8.60
Old solutions at renewal: 7.51 - 8.30
Nominal and measured concentrations:
Nominal test concentrations: control, 2.5, 5, 10, 20 and 40 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass beakers covered with transparent lids
- Size, fill volume: 150 mL, 50 mL
- Aeration: no
- Renewal rate of test solution: renewal of old solutions for fresh solutions after 24 h of exposure
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h darkness

Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.162 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
1.77 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
2.87 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.034 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- In samples of fresh solutions the concentration of the test item was in the range of 80.0 – 92.8% of nominal.
- In samples of old solutions the determined concentration of the detected substance was in the range of 25.0 – 82.2% of initial.
- The concentration of the test item was not stable under test conditions.
Results with reference substance (positive control):
The EC50 (48h) of the control substance potassium dichromate was 0.723 mg/L.

Immobilization of Daphnia magna in control was 5% (allowed not more than 10%).

Oxygen concentration in the test vessels was between 7.07 – 8.60 mg/L (allowed not less than 3 mg/L).

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of aquatic invertebrates was investigated for ‘α,α'-propylenedinitrilodi-o-cresol’ with the waterflea Daphnia magna in a GLP guideline study according to OECD 202. 

After 48 hours exposure in a semi-static test system the EC50 was 3.162 mg/L based on measured test substance concentration.

Description of key information

Acutely toxic to aquatic invertebrates.

The acute toxicity of aquatic invertebrates was investigated with the waterflea Daphnia magnas in a GLP guideline study according to OECD 202.

After 48 hours exposure in a semi-static test system the EC50 was 3.162 mg/l based on measured test substance concentration.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
3.162 mg/L

Additional information