Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-278-6 | CAS number: 118-79-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,4,6-tribromophenol
- EC Number:
- 204-278-6
- EC Name:
- 2,4,6-tribromophenol
- Cas Number:
- 118-79-6
- Molecular formula:
- C6H3Br3O
- IUPAC Name:
- 2,4,6-tribromophenol
- Test material form:
- solid: granular
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: MANAC, Hiroshima, JAPAN; Lot No.: 70909
- Purity test date: 99.8 %
- Storage conditions: cold storage, closed vessel, in the dark
Method
- Target gene:
- His
Trp
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9; liver homogenate obtained from male rats after induction with phenobarbital and benzoflavone
- Test concentrations with justification for top dose:
- Preliminary test to find the cytotoxic concentration:
- without S9 mix:
500 µg/plate and greater for TA100, TA1535, and TA98
1500 µg/plate and greater for TA 1537
5000 µg/plate for WP2 urvA
- with S9 mix:
500 µg/plate and greater for TA100, TA1535, and TA1537
1500 µg/plate and greater fot TA 98
5000 µg/plate for WP2 urvA
Main test concentration:
- without S9 mix:
0, 15.6, 31.3, 62.5, 125, 250, 500 µg/plate for S. typhimurium TA100, TA1535, and TA98
0, 31.3, 62.5, 125, 250, 500, 1000 µg/plate for S. typhi. TA1537
0, 156, 313, 625, 1250, 2500, 5000 µg/plate for E.coli. WP2 urvA
- with S9 mix:
0, 15.6, 31.3, 62.5, 125, 250, 500 µg/plate for S. typhimurium TA100, TA1535, and TA1537
0, 15.6, 31.3, 62.5, 125, 250, 500, 1000 µg/plate for S. typhimurium TA 98
0, 156, 313, 625, 1250, 2500, 5000 µg/plate for E. coli. WP2 urvA - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- 9-aminoacridine
- sodium azide
- other: +S9 mix: 2-Aminoanthracene for WP2 uvrA, TA1535, TA1537, TA100, TA98
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours at 37°C
DETERMINATION OF CYTOTOXICITY
- Method: stereomicroscope to judge condition of bacterial membrane on agar surface - Evaluation criteria:
- A positive (mutagenic) response in this test system is defined as the average number of colonies on the plate containing the test material being more than twice the number growing on plates containing solvent only in at least one of the bacterial strains. The response should be reproducible and dose dependent.
- Statistics:
- The average and standard deviation of each dose level were calculated.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Growth inhibition was observed at 500 µg/plate.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Growth inhibition was observed at 500 µg/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Growth inhibition was observed at 500 µg/plate.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Growth inhibition was observed at 500 µg/plate.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Growth inhibition was observed at 2500 µ/plate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The results showed that the increase of the number of revertant colonies of all strains against the solvent control did not exceed the 2-fold criterion for a positive result, and therefore the test results were negative.
Applicant's summary and conclusion
- Conclusions:
- No increase in mutation frequency was detected in this study using the criterion that a positive result would be defined as a two-fold increase in colonies compared to the solvent control. All positive controls increased mutation frequency, and the solvent control fell within historical levels.
2,4,6 -tribromophenol is not mutagenic according to the conditions of this study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
