2,4,6-tribromophenol

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-tribromophenol

EC Number:

204-278-6

EC Name:

2,4,6-tribromophenol

Cas Number:

118-79-6

Molecular formula:

C6H3Br3O

IUPAC Name:

2,4,6-tribromophenol

Test material form:

solid: granular

Test animals

Species:

rabbit

Strain:

other: Local strain of albino rabbits; not further specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: local strain obtained from A. Loebenstein Laboratory Animals, Yoqneam
- Age at study initiation: no information
- Weight at study initiation: 2.1 to 2.7 grams
- Housing: individual aluminum cages
- Diet (e.g. ad libitum): Commercially available complete pelleted rabbit diet (Arnavit), ad libitum
- Water: tap water, ad libitum
- Acclimation period: 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 +/- 2 °C
- Humidity (%): 60% +/- 15%
- Air changes (per hr): 17
- Photoperiod (hrs dark / hrs light): 14 hours light per day

Test system

Type of coverage:

occlusive

Preparation of test site:

clipped

Controls:

no

Amount / concentration applied:

500 mg

Duration of treatment / exposure:

4h

Observation period:

5, 24, 48 and 72 hours

Number of animals:

6

Details on study design:

Single doses (0.5 g) of the test material were placed on to unmedicated gauze patches (25 x 25 mm) and were applied to the skin test site. The patch was held in place by a porous dressing strip which was then kept in place by an adhesive bandage to ensure that the test material was in close contact with the skin. The use of impervious material and occlusive dressing was to maximize the dermal absorption of the test material by the skin.

TEST SITE
- Area of exposure: Dorsum between the limb girdles
- % coverage: 25 x 25 mm
- Type of wrap if used: unmedicated gauze patches applied to skin and held in place by a porous dressing strip and adhesive bandage

REMOVAL OF TEST SUBSTANCE
- Washing (if done): gently wiped using double-distilled water to remove excess test material
- Time after start of exposure: 4 hours

SCORING SYSTEM: Criteria of Draize (1959) was used.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0.16

Reversibility:

not fully reversible within: 24 h

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Irritant / corrosive response data:

One rabbit had a very slight erythema 5 and 24 hours after dosing.

Animal No. Type of Score at time after dosing
and sex response 5 hrs 24 hrs 48 hrs 72 hrs
------------------------------------------------------------------------------------------
1 male E/O a) 0,0 0,0 0,0 0,0
2 male E/O a) 1,0 1,0 0,0 0,0
3 male E/O a) 0,0 0,0 0,0 0,0
1 female E/O a) 0,0 0,0 0,0 0,0
2 female E/O a) 0,0 0,0 0,0 0,0
3 female E/O a) 0,0 0,0 0,0 0,0
a) E/O: Erythema/Oedema (respectively)

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Under the conditions of this study, Tribromophenol is assessed as being a non-irritant material to the rabbit skin.