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Diss Factsheets
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EC number: 428-040-8 | CAS number: 138261-41-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://www.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 Jun to 25 Jun, 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The measured concentrations of the test material in test dilution water were determined at 0- and 48-hours of the toxicity test. Control and the test material fortified samples were also determined at each sampling period.
During the 0-hour sampling, 40 mL aliquots from parent solutions were sampled. At the 48-hour sampling period, 20 ml aliquots from each replicate, for a total of 40 mL were sampled. - Vehicle:
- no
- Details on test solutions:
- The preparation of the test solutions was corrected for the specified purity. This material was used to prepare quality control samples. The highest test concentration (113 mg/L) was sonicated for approximately 20 minutes to break up some precipitate that had formed when adding the compound to the dilution water.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The Daphnia magna used in the test were cultured at the ABC facilities. The adult daphnids were fed with a suspension of at least one algae species Selenastrum capricornutum, Ankistrodesmus falcatus and/or Chlamydomonas reinhardtii. Along with the algae, the daphnids were fed with a supplement consisting of trout chow and yeast. The daphnids were fed with this feeding regime at least every three days. The original culture of daphnids at ABC Labs were identified to species using the taxonomic key presented by Pennak. A total of 120 instar from ABC culture were used for definitive bioassay.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 140 mg/L
- Test temperature:
- 20-21°C
- pH:
- 8.3 - 8.4
- Dissolved oxygen:
- 8.0 - 8.4 mg/mL (94 - 97% saturation)
- Salinity:
- Not applicable
- Conductivity:
- 310 µmhos/cm
- Nominal and measured concentrations:
- Nominal: 16, 27, 45, 75 and 125 mg/L
Measured: 15, 25, 42, 71 and 113 mg/L - Details on test conditions:
- The bioassay was conducted in 250 mL glass beakers containing 200 mL of daphnid culture/test water. The depth and diameter of the test vessels were approximately 9 and 6.5 cm, respectively. The depth and surface diameter of the test solution in the test vessels was approximately 6.3 and 6.5 cm, respectively. All test vessels were covered with loose-fitting petri dish covers to minimize evaporation and prevent contamination during the study. These vessels were kept at 20 ±1.0°C in a temperature controlled water bath with a continuous recording of the water bath temperature maintained during the study. The lighting was maintained at 50-70 footcandles on a 16-hour daylight photoperiod with 30-minute simulated dawn and dusk periods.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 85 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- The no-effect concentration based on the absence of immobility and abnormal effects was 42 mg/L since no abnormal effects were observed at or below this concentration after 48 hours. The abnormal effects of immobility, daphnids coated with extraneous material and/or daphnids on the bottom of test chambers were observed in the mean measured concentrations of 71 and 113 mg/L during the study.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The present guideline study was conducted in compliance with GLP. Under the test conditions used, the 48-hour EC50 for Daphnia magna was 85 mg/L.
Reference
Please refer to "overall remark/ attached background material" field for result tables.
Description of key information
From the acute toxicity study of the test substance on Cloeon dipterum the 48-hours EC50 for aquatic invertebrates was 2.7 µg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.7 µg/L
Additional information
In the key study (1990), the acute toxicity of test material to aquatic invertebrates was investigated in a 48-hours static test with the Daphnia magna according to guideline EPA No. 72-2. Ten daphnia per replicate and 2 replicates per test concentration were exposed for 48 hours under static conditions to mean measured concentrations of 15, 25, 42, 71 and 113 mg a.s./L, alongside with a control. The 48-hour EC50 was determined to be 85 mg a.s./L
A non-guideline study on acute toxicity of the test substance on Cloeon dipterum is available. The EC50 (48 h) value was determined to be 0.0027 mg/L. This study was considered relevant and reliable for use in classification in accordance with RAC “Opinion proposing harmonised classification and labelling at EU level of imidacloprid”and will therefore be used for the PNEC derivation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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