Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

The widespread use of enzymes has been followed by investigations relevant for ecotoxicity. Investigations are mainly performed to establish knowledge on short-term aquatic toxicity and on the rate of biodegradability of the enzyme. Algae growth inhibition tends to be the most sensitive aquatic endpoint reported for enzyme preparations  in the literature with EC50 concentrations ranging from 99 to >1,000 mg/L [2].  The published data support that enzyme preparations are not highly inhibitory to the growth of aquatic algae and non-proteolytic enzymes, such as monoamine oxidase, should not be classified as harmful or toxic to the environment [1].


Algae appears to be the most sensitive species and the EC50 value of 99 mg/L for lipases was used for conservative PNEC derivations.


[1] Enzymes REACH Consortium (2010). Data waiving argumentation for technical enzymes.

[2] HERA Human and environmental risk assessment on ingredients of household cleaning products - alpha-amylases, cellulases and lipases. 2005.

Additional information