1-chloro-4-nitrobenzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

: no pathologic examination performed, no individual animal data, lack of details on test substance

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-nitrobenzene

EC Number:

202-809-6

EC Name:

1-chloro-4-nitrobenzene

Cas Number:

100-00-5

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): p-Nitrochlorbenzol
- Analytical purity: no data

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann, Germany
- Weight at study initiation: 160 - 180 g
- Housing: 5 in one cage

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Details on oral exposure:

no data

Doses:

100, 200, 300, 350, 400, 500, 600 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Statistics:

LD50 was calculated by Probit-analysis (Fink and Hund 1965. Arzneim.-Forsch. 15:624).

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

294 mg/kg bw

Mortality:

200 mg/kg bw: 3/10 animals died 2d after application
300 mg/kg bw: 5/10 animals died 2-3d after application
350 mg/kg bw: 5/10 animals died 2-3d after application
400 mg/kg bw: 8/10 animals died 2-4d after application
500 mg/kg bw: 8/10 animals died 2-3d after application
600 mg/kg bw: 10/10 animals died 3h-4d after application

Clinical signs:

other: reduced general condition, cyanotic appearance, diarrhea, increased excretion of urine

Gross pathology:

no data

Any other information on results incl. tables

Table 1:

Dose (mg/kg bw)	Time of death	Dead	Symptoms	Number of rats used
100 200 300 350 400 500 600	- 2d 2-3d 2-3d 2-4d 2-3d 3h-4d	0 3 5 5 8 8 10	0 10 10 10 10 10 10	10 10 10 10 10 10 10

Value: (232 - 349 mg/kg bw)

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

Classification:
GHS: Acute Oral Category 3

Executive summary:

Loeser (Bayer AG), 1979

The acute oral toxicity of 1 -chloro-4 -nitrobenzene was investigated in male Wistar II rats comparable to OECD guideline 401.

7 male groups of 10 animals were dosed with 100, 200, 300, 350, 400, 500 and 600 mg/kg bw 1-chloro-4 -nitrobenzene per gavage and observed for 14 days following exposure for mortality and clinical signs. Mortalities occurred at dose levels equal to and exceeding 200 mg/kg bw 2 days after administration. Reduced general condition, cyanotic appearance, diarrhea, increased excretion of urine were observed in all male animals dosed 200 - 600 mg/kg bw. Symptoms started to appear between 3 hours (600 mg/kg bw) and 4 days (400 and 600 mg/kg bw) after administration. No clinical signs were observed in animals dosed with 100 mg/kg bw in male rats. The calculated LD50 for male rats was 294 mg/kg bw.