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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Results of the harmful effects of water pollutants to green algae (Scenedesmus subspicatus) in the cell multiplication inhibition test
Author:
Kuehn R, Pattard M
Year:
1990
Bibliographic source:
Wat. Res. 24, 31-38
Reference Type:
secondary source
Title:
1-CHLORO-4-NITROBENZENE CAS No: 100-00-5 SIDS Initial Assessment Report.
Author:
OECD
Year:
2002
Bibliographic source:
UNEP Publications

Materials and methods

Principles of method if other than guideline:
DIN-Standard 38412 L9 (Algae, Cell multiplication inhibition test), Method of the German Standards Institution, Berlin, Germany
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chloro-4-nitrobenzene
EC Number:
202-809-6
EC Name:
1-chloro-4-nitrobenzene
Cas Number:
100-00-5
Molecular formula:
C6H4ClNO2
IUPAC Name:
1-chloro-4-nitrobenzene
Details on test material:
- Name of test material (as cited in study report): 1-chloro-4-nitrobenzene
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Before beginning the test, the stock solution of the test substance (sample) was adjusted to pH.

Test solutions

Details on test solutions:
The test substance was quantitatively dissolved to produce an stock solution in 800 mL double
distilled water.
Wide-neck bottles of 250mL with ground-glass stoppers were used as the test vessels.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
Stock culture:
- Scenedesmus subspicatus (strain number 8681 SAG)
- 100 mL flasks with caps and 20 mL nutrient solution sterilized and inoculated with 2 mL
cell suspension taken from a 10-day old culture.

Preliminary culture:
- The cell concentration in the preliminary culture flasks must amount to 10x E4 mL-1. This was to ensure that the culture was still in a process of logarithmic growth after 72 h.
- The cell material of the preliminary cultures was used after 72 h to inoculate the dilution preparation after the cell concentration had been fixed at 10xE5 mL-1.
- The cultivation of the preliminary cultures was undertaken 3 days prior to the preparation of the test solution.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
21-25°C
pH:
8 ± 0.3
Nominal and measured concentrations:
Tested concentration range (mg/L; nominal): 0.4-50
Details on test conditions:
The test and control preparations were incubated under constant lighting and shaken daily.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
4.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Conclusions:
A cell multiplication test in accordance with DIN 38412, L9 (German Institute of Standardization) was performed with the test substance 1-chloro-4-nitrobenzene. A 48h-ErC50 (related to nominal concentration) of 16 mg/L for Scenedesmus subspicatus was determined. The results of the test can be considered reliable.
Executive summary:

Scenedesmus subspicatus proved to be a sensitive freshwater species in a cell multiplication inhibition test in accordance with DIN 38412, L9 (German Institute of Standardization). A 48 h-EC50 value (related to nominal concentration) of 16 mg/litre (growth rate) for Scenedesmus subspicatus was determined (Kuehn and Pattard, 1990).