Anthranilamide

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-guideline study available as unpublished report, limitations in design and/or reporting but otherwise adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

According to BASF-internal standard. Five Kisslegg mice per sex per dose were intraperitoneally exposed to 800, 1000, 1250, 1600, 2000, 2500, and 3200 mg/kg of the test substance followed by a 7 day observation period. Clinical signs were recorded and macroscopic examinations were performed.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anthranilamide

EC Number:

201-851-2

EC Name:

Anthranilamide

Cas Number:

88-68-6

Molecular formula:

C7H8N2O

IUPAC Name:

2-aminobenzamide

Details on test material:

Name of the test substance used in the study report: Anthranilsaeureamid

Test animals

Species:

mouse

Strain:

other: Kisslegg mice

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Details on exposure:

The test concentration used was 16% (w/v) in aqueous suspension with CMC.

Doses:

800, 1000, 1250, 1600, 2000, 2500, 3200 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, macroscopic examination.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 064 mg/kg bw

Based on:

test mat.

Remarks on result:

other: (947 - 1197 mg/kg)

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

1 010 mg/kg bw

Based on:

test mat.

Remarks on result:

other: (828 - 1233 mg/kg)

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

1 106 mg/kg bw

Based on:

test mat.

Remarks on result:

other: (958 - 1278 mg/kg)

Mortality:

- 800 mg/kg bw: 1 female rat died within 48 hours and another male rat within 7 days
- 1000 mg/kg bw: 1 male rat died within 24 hours and another male rat died within 7 days
- 1250 mg/kg bw: 4 males and 3 females within 24 hours. In addition, 1 male rat died within 48 hours and 1 female died within 7 days.
- 1600 mg/kg: 4 males and 5 females within 24 hours.
- 2000 - 3000 mg/kg: 10/10 within 24 hours.

Clinical signs:

- 1600-3200 mg/kg bw: Immediately after injection, lying in abdominal and lateral position, rapid breathing and apathy was observed. No symptoms were observed after 4 days.
- 800-1250 mg/kg bw: Immediately after injection, staggering and 5 minutes later lying abdominal and lateral position and rapid breathing was observed. No symptoms were observed after 4 days

Gross pathology:

Congestive hyperemia of the liver was observed.